Characterization of the mouse olfactory glutathione S-transferases during the acute phase response.
Characterization of the mouse olfactory glutathione S-transferases during the acute phase response.
复制标题
急性期反应期间小鼠嗅觉谷胱甘肽 S-转移酶的表征。
DOI：
10.1002/jnr.10687
复制
发表时间：
2003
影响因子：
4.2
通讯作者：
Walters,Eric
中科院分区：
文献类型：
--
作者：
Weech,Michelle;Quash,Michelle;Walters,Eric
急性期反应（APR）已被证明可以改变生物转化酶的表达和活性，如I期细胞色素P450和II期谷胱甘肽S转移酶（GSTs）。细胞色素P450和GSTs在嗅粘膜的非神经元细胞中大量表达和共定位。先前的研究表明，嗅觉细胞色素P450的表达和活性在局部炎症和感染期间发生改变。然而，人们对APR对嗅觉GST酶的影响知之甚少。本研究研究了急性期诱诱剂多肌苷-多胞苷酸（polyIC）处理24小时后，APR对小鼠嗅粘膜GST同工酶表达和活性的影响。针对特异性GST亚型α （A1‐1）、μ （M1‐1）和π （P1‐1）的抗体进行的Western blot分析表明，它们的表达在polyIC处理下没有改变。相比之下，嗅觉P450 2E1的表达显著降低。嗅觉gst对一般底物1‐氯‐2,4‐二硝基苯（CDNB）的酶活性在APR过程中没有变化。嗅觉谷胱甘肽含量分析表明，APR过程中嗅觉谷胱甘肽含量也不受polyIC的影响。在APR期间，这些嗅觉GST异构体的不敏感性可能在限制感染和炎症对嗅觉系统的影响方面发挥重要作用。©2003 Wiley‐Liss, Inc。
The acute phase response (APR) has been shown to alter expression and activity of biotransformation enzymes, such as the phase I cytochromes P450 and phase II glutathione S‐transferases (GSTs). The cytochromes P450 and GSTs are expressed abundantly and colocalized to non‐neuronal cells of the olfactory mucosa. Previous studies indicate that olfactory cytochromes P450 expression and activity is altered during periods of localized inflammation and infection. Little is understood, however, about the influence of the APR on olfactory GST enzymes. This study investigated effects of the APR on olfactory GST isozymes expression and activity in mouse olfactory mucosa after 24‐hr treatment with the acute phase inducer, polyinosinic: polycytidylic acid (polyIC). Western blot analysis using antibodies directed against specific GST isoforms α (A1‐1), μ (M1‐1), and π (P1‐1) demonstrated that their expression was unaltered by polyIC treatment. In contrast, olfactory P450 2E1 expression was significantly decreased. Enzymatic activity of the olfactory GSTs toward the general substrate, 1‐chloro‐2,4‐dinitrobenzene (CDNB) was unchanged during the APR. Analysis of olfactory glutathione content during the APR showed that it was also unaffected by polyIC. The insensitivity of these olfactory GST isoforms during the APR may play a significant role toward limiting the impact of infection and inflammation on the olfactory system. © 2003 Wiley‐Liss, Inc.