Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling
Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling
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DOI：
10.1002/glia.23613
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发表时间：
2019-07-01
期刊：
影响因子：
6.2
通讯作者：
Lohr, Christian
中科院分区：
文献类型：
--
作者：
Beiersdorfer, Antonia;Scheller, Anja;Lohr, Christian
星形胶质细胞排列在高度组织化的间隙连接耦合网络中，通过Ca2+波的传播进行通信。星形胶质细胞不仅与相邻的星形胶质细胞，而且与少突胶质细胞间隙连接偶联，形成所谓的神经节合胞体。然而，目前尚不清楚是否在panglial合胞体的神经胶质细胞传输信息，使用Ca2+信号。我们使用共聚焦钙离子成像研究星形胶质细胞和嗅鞘神经胶质细胞（OECs）之间的细胞间通讯的小鼠嗅球的整体准备。我们的研究结果表明，Ca2+瞬变的肾小球星形胶质细胞，诱发局部光解的“笼”ATP和“笼”tACPD，导致随后的Ca2+反应的OECs。这种从星形胶质细胞到嗅鞘细胞的Ca 2+反应的传输在神经元抑制的存在下持续存在，但当用甘珀酸抑制间隙连接偶联时不存在。当Ca2+瞬变直接诱发嗅鞘细胞的喷应用DHPG，他们导致延迟的Ca2+反应在肾小球星形胶质细胞，表明panglial传输的Ca2+信号发生在双向的方式。此外，从星形胶质细胞到嗅鞘细胞的Ca2+信号的panglial传递导致嗅神经层中嗅鞘细胞相关血管的血管收缩。我们的研究结果表明，在间隙连接耦合panglial网络和由此产生的调节血管直径的嗅球中的不同类别的胶质细胞之间的钙离子信号的功能传输。
Astrocytes are arranged in highly organized gap junction-coupled networks, communicating via the propagation of Ca2+ waves. Astrocytes are gap junction-coupled not only to neighboring astrocytes, but also to oligodendrocytes, forming so-called panglial syncytia. It is not known, however, whether glial cells in panglial syncytia transmit information using Ca2+ signaling. We used confocal Ca2+ imaging to study intercellular communication between astrocytes and olfactory ensheathing glial cells (OECs) in in-toto preparations of the mouse olfactory bulb. Our results demonstrate that Ca2+ transients in juxtaglomerular astrocytes, evoked by local photolysis of "caged" ATP and "caged" tACPD, led to subsequent Ca2+ responses in OECs. This transmission of Ca2+ responses from astrocytes to OECs persisted in the presence of neuronal inhibition, but was absent when gap junctional coupling was suppressed with carbenoxolone. When Ca2+ transients were directly evoked in OECs by puff application of DHPG, they resulted in delayed Ca2+ responses in juxtaglomerular astrocytes, indicating that panglial transmission of Ca2+ signals occurred in a bidirectional manner. In addition, panglial transmission of Ca2+ signals from astrocytes to OECs resulted in vasoconstriction of OEC-associated blood vessels in the olfactory nerve layer. Our results demonstrate functional transmission of Ca2+ signals between different classes of glial cells within gap junction-coupled panglial networks and the resulting regulation of blood vessel diameter in the olfactory bulb.