A 70 amino acid region within the semaphorin domain activates specific cellular response of semaphorin family members
A 70 amino acid region within the semaphorin domain activates specific cellular response of semaphorin family members
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DOI：
10.1016/s0896-6273(00)80369-4
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发表时间：
1997-09-01
期刊：
影响因子：
16.2
通讯作者：
Raper, JA
中科院分区：
文献类型：
--
作者：
Koppel, AM;Feiner, L;Raper, JA
信号素家族包含分泌和跨膜信号蛋白，在神经、免疫和心血管系统中发挥作用。鸡崩解蛋白-1是一种对特定生长锥体的驱避剂。信号素家族的另外两个分泌成员，崩溃蛋白-2和崩溃蛋白-3，在结构上与崩溃蛋白-1相似，但具有不同的生物活性。信号素含有一个由500个氨基酸组成的家族标志性信号素结构域。我们在本研究中表明：(1)崩溃蛋白-1的信号素结构域是生物活性的必要条件和充分条件，(2)信号素结构域包含一个70个氨基酸的区域，指定这三个家族成员的生物活性，(3)带正电荷的羧基末端增强活性而不影响特异性。我们认为，信号素通过两个独立的结合位点与其受体相互作用：一个介导生物反应，另一个增强生物反应。
The semaphorin family contains secreted and transmembrane signaling proteins that function in the nervous, immune, and cardiovascular systems. Chick collapsin-1 is a repellent for specific growth cones. Two other secreted members of the semaphorin family, collapsin-2 and -3, are structurally similar to collapsin-1 but have different biological activities. Semaphorins contain a 500 amino acid family signature semaphorin domain. We show in this study that (1) the semaphorin domain of collapsin-1 is both necessary and sufficient for biological activity, (2) the semaphorin domain contains a 70 amino acid region that specifies the biological activity of the three family members, and (3) the positively charged carboxy terminus potentiates activity without affecting specificity. We propose that semaphorins interact with their receptors through two independent binding sites: one that mediates the biological response and one that potentiates it.