Subcellular localization of superoxide dismutase in rat liver.
Subcellular localization of superoxide dismutase in rat liver.
复制标题
大鼠肝脏中超氧化物歧化酶的亚细胞定位。
DOI：
10.1042/bj1500031
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
P. Baudhuin
中科院分区：
文献类型：
--
作者：
C. Peeters;A. Vandevoorde;P. Baudhuin
研究了超氧化物歧化酶在大鼠肝匀浆中的亚细胞定位。大部分超氧化物歧化酶活性存在于可溶性部分中（84%），其余部分与线粒体相关。没有迹象表明超氧化物歧化酶的发生在其他亚细胞结构，特别是在过氧化物酶体，被发现。线粒体的活动是不是由于吸附，因为sedimentable活动基本上是潜在的。亚分馏的线粒体低渗休克和超声处理表明，一半的线粒体超氧化物歧化酶活性定位在膜间隙，其余的酶是一个组成部分的基质空间。然而，在非离子介质中，基质酶被吸附到内膜上，它可以被低浓度（0.04M）的KCl解吸。超氧化物歧化酶的活性被发现在所有的大鼠器官调查。在肝脏、肾上腺和肾脏中观察到该酶的最大活性。在肾上腺中，最高的比活度与髓质有关。
The subcellular localization of superoxide dismutase was investigated in rat liver homogenates. Most of the superoxide dismutase activity is present in the soluble fraction (84%), the rest being associated with mitochondria. No indications for the occurrence of superoxide dismutase in other subcellular structures, particularly in peroxisomes, was found. Mitochondrial activity is not due to adsorption, since the sedimentable activity is essentially latent. Subfractionation of mitochondria by hypo-osmotic shock and sonication shows that half of the mitochondrial superoxide dismutase activity is localized in the intermembrane space, the rest of the enzyme being a component of the matrix space. In non-ionic media the matrix enzyme is, however, adsorbed to the inner membrane, from which it can be desorbed by low (0.04M) concentration of KCl. Superoxide dismutase activity was found in all rat organs investigated. Maximal activity of the enzyme is observed in liver, adrenals and kidney. In adrenals, the highest specific activity is associated with the medulla.