All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters.
All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters.
复制标题
DOI：
10.1038/srep20682
复制
发表时间：
2016-02-09
影响因子：
4.6
通讯作者：
Carabelli V
中科院分区：
文献类型：
--
作者：
Picollo F;Battiato A;Bernardi E;Plaitano M;Franchino C;Gosso S;Pasquarelli A;Carbone E;Olivero P;Carabelli V
报道了用离子束技术在单晶金刚石（SCD）衬底上制备16个石墨微通道的全碳多电极阵列（MEA）。所制造的SCD-MEA系统地用于在体外同时安培检测嗜铬细胞群体的分泌活性，展示了一种新的传感方法相对于标准技术。基于SCD的设备的生物化学稳定性和生物相容性与多电极阵列的并行记录相结合，允许：i）在大量细胞的药物筛选和/或药理学测试期间，在数据收集方面显著节省时间，和iii）用完全无毒和化学上坚固的生物敏感底物在许多循环中重复进行采集。
We report on the ion beam fabrication of all-carbon multi electrode arrays (MEAs) based on 16 graphitic micro-channels embedded in single-crystal diamond (SCD) substrates. The fabricated SCD-MEAs are systematically employed for the in vitro simultaneous amperometric detection of the secretory activity from populations of chromaffin cells, demonstrating a new sensing approach with respect to standard techniques. The biochemical stability and biocompatibility of the SCD-based device combined with the parallel recording of multi-electrodes array allow: i) a significant time saving in data collection during drug screening and/or pharmacological tests over a large number of cells, ii) the possibility of comparing altered cell functionality among cell populations, and iii) the repeatition of acquisition runs over many cycles with a fully non-toxic and chemically robust bio-sensitive substrate.