Activin subunit, follistatin, and activin receptor gene expression in the prepubertal female rat pituitary.
Activin subunit, follistatin, and activin receptor gene expression in the prepubertal female rat pituitary.
复制标题
青春期前雌性大鼠垂体中的激活素亚基、卵泡抑素和激活素受体基因表达。
DOI：
10.1095/biolreprod59.2.278
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发表时间：
1998
影响因子：
3.6
通讯作者：
Handa,RJ
中科院分区：
文献类型：
--
作者：
Wilson,ME;Handa,RJ
在青春期前的雌性大鼠中，垂体腺的FSH分泌和mRNA表达在出生后第二周即将结束时出现一过性和选择性的增加。为了研究激活素在此期间上调促性腺激素的可能性，我们研究了激活素β亚基、卵泡抑素和激活素受体亚型在青春期前雌性大鼠脑垂体中的表达。用半定量逆转录聚合酶链式反应方法检测βA、βB和卵泡抑素mRNAs在出生后第8、10、12、15、21天的脑垂体中的表达水平。所有的值都与在发情间期被杀的成年雌性的值进行了比较。βA亚基基因表达水平在出生后所有日龄均显著升高(P<0.05)，βB基因表达水平仅在出生后第10天高于成人水平(P<；0.05)。卵泡抑素基因在第8天开始升高(p<；0.05)，之后逐渐下降至成体水平。用原位杂交法检测激活素受体II型亚型mRNAs的表达和分布。激活素受体II型(ActRII)的mRNA表达广泛表达于脑垂体的各个区域。另一方面，激活素受体IIB型(ActRIIB)在垂体前叶细胞亚群中高表达。激活素受体亚型mRNAs原位杂交结合免疫细胞化学检测含FSH细胞。结果表明，在幼年女性脑垂体中，FSH免疫反应阳性细胞中一般不表达Act RII基因，而FSH免疫反应细胞中表达Act RIIB基因。在第10天和第15天，与第21天相比，第10天和第15天的ACT RII基因表达水平较低(P<；0.05)，而ACT RIIB基因的表达不随年龄的增长而变化。这些数据表明，激活素调节系统的基本成分存在于婴儿的雌性脑垂体腺中，因此可能参与了此时FSH的不同调节。
In the prepubertal female rat, a transient and selective increase in FSH secretion and mRNA expression by the pituitary gland occurs toward the end of the second postnatal week of life. To begin to investigate the possibility that activin may play a role in up-regulating FSH during this time, we have studied the ontogeny of the expression of the activin β subunits, follistatin, and activin receptor subtypes in the prepubertal female rat pituitary. The levels of expression of βA, βB, and follistatin mRNAs were determined in the pituitary gland on postnatal days (PND) 8, 10, 12, 15, and 21 by semiquantitative reverse transcription-polymerase chain reaction. All values were compared to those of adult females killed on diestrus. mRNA levels of subunit βAwere significantly (p< 0.05) elevated on all postnatal days examined; βBmRNA levels were elevated above adult levels only on PND 10 (p< 0.05). Follistatin mRNA was high on PND 8 (p< 0.05) and then decreased to adult levels. The level and distribution of activin receptor type II subtype mRNAs were determined by in situ hybridization. Activin receptor type II (Act RII) mRNA expression was diffusely expressed throughout all areas of the pituitary. Activin receptor type IIB (Act RIIB), on the other hand, was highly expressed by a subset of anterior pituitary cells. In situ hybridization for activin receptor subtype mRNAs was combined with immunocytochemistry to detect FSH-containing cells. We determined that in the infantile female pituitary, Act RII mRNA was generally not expressed in FSH-immunoreactive cells, while Act RIIB mRNA was expressed in FSH-immunoreactive cells. Act RII mRNA was lower on PND 10 and 15 when compared to PND 21 (p< 0.05), whereas Act RIIB mRNA expression did not change with age. These data suggest that the essential components of the activin regulatory system are present in the infantile female pituitary gland and thus may be involved in the differential regulation of FSH at this time.