Immunohistochemical and in situ hybridization studies of the liver and kidney in human leptospirosis
Immunohistochemical and in situ hybridization studies of the liver and kidney in human leptospirosis
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DOI：
10.1007/s00428-006-0163-z
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发表时间：
2006-05-01
期刊：
影响因子：
3.5
通讯作者：
Alves, V. A. F.
中科院分区：
文献类型：
--
作者：
De Brito, T.;Menezes, L. F.;Alves, V. A. F.
应用原位杂交技术(ISH)检测组织中钩端螺旋体DNA，并与免疫组织化学(IHC)相结合，对福尔马林固定、石蜡包埋的人钩端螺旋体病肝肾标本进行了诊断和致病作用的评价。抗E-钙粘素抗体的IHC检测评估了钩端螺旋体病中常见的肝板紊乱。免疫组织化学检测到钩端螺旋体抗原(LAG)在巨噬细胞中，在人的肝脏和肾脏中都有。在豚鼠中，除了这些发现，还发现肝细胞的细胞膜上有染色，偶尔在肾小管上皮细胞的顶膜上也有染色。ISH阳性信号主要见于人肝细胞胞核和实验性感染豚鼠肝细胞的胞浆和胞核。E-钙粘素膜表达缺失与肝板紊乱有关。对这些发现进行了讨论，认为在钩端螺旋体病中，细胞膜损伤可能在该病的发病机制中起重要作用。最后，有人建议，IHC和/或ISH可同时用于诊断和研究目的。
An in situ hybridization (ISH) assay for the detection of leptospiral DNA in tissues was described and its diagnostic and pathogenetic usefulness in combination with immunohistochemistry (IHC) was evaluated in formalin-fixed, paraffin-embedded liver and kidney samples from human fatal cases of leptospirosis. IHC assays with anti-E-cadherin antibodies assessed the liver-plate disarray frequently observed in leptospirosis. Immunohistochemistry detected leptospiral antigen (LAg) in macrophages, both in human liver and kidney. In guinea pigs, in addition to these findings, staining on cell membranes of hepatocytes and, occasionally, in apical membrane of kidney tubular cells was demonstrated. Positive ISH signal was observed chiefly in the nuclei of human hepatocytes and in the cytoplasm and nuclei of liver cells of experimentally infected guinea pigs. Loss of E-cadherin membrane expression is associated with liver-plate disarray. These findings were discussed in the contention that, in leptospirosis, cell membrane damage might be important for the pathogenesis of the disease. Finally, it was suggested that both IHC and/or ISH might be used for both diagnostic and research purposes.