Comprehensive analysis of wintersweet flower reveals key structural genes involved in flavonoid biosynthetic pathway
Comprehensive analysis of wintersweet flower reveals key structural genes involved in flavonoid biosynthetic pathway
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腊梅花综合分析揭示类黄酮生物合成途径关键结构基因
DOI：
10.1016/j.gene.2018.08.050
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Longqing Chen
中科院分区：
文献类型：
--
作者：
Nan Yang;Kaige Zhao;Xiang Li;Rong Zhao;Muhammad z Aslam;Li Yu;Longqing Chen
蜡梅（Chimonanthus praecox（L.）），在我国有着上千年的栽培历史，至今仍是一种广受欢迎的观赏木本植物。蜡梅花的花被片呈蜡状，花的整体颜色为黄色，而内部花被片的颜色从黄色到红色不等，这使其成为研究观赏灌木花色形成的理想植物。HPLC分析表明，花被片中的主要色素是黄酮类化合物的代谢产物。所有花被片中均含有槲皮素、山萘酚3-O-芸香糖苷和芦丁，而矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-芸香糖苷仅在红色花被片中发现。此外，我们发现芦丁是所有色素的主要成分。本研究还构建了两个品种H29和H64花的参考转录组文库。进一步利用蛋白质组学技术对H29花中的30个类黄酮合成途径蛋白进行了鉴定。基于这些数据，还推测了黄酮类化合物的生物合成途径。通过基因表达定量分析，我们发现ANS作为红色素积累的开关，与类黄酮途径的各个步骤基因呈正相关。这种表达谱表明，没有基因产物竞争共同的底物来重定向蜡梅的代谢通量。F3 ′ H的高表达为黄酮醇和花青素的生物合成提供了充足的前体二氢槲皮素。研究结果有助于深化和丰富蜡梅花色形成的基因资源，为增加花色多样性提供具体的育种策略。
Wintersweet (Chimonanthus praecox(L.)), with an over-one-thousand-years long history in cultivation, is still a popular ornamental woody plant in China. The tepals of wintersweet flower are waxy in nature and the overall color of the flower is yellow, while the inner tepals range from yellow to red, which makes it an ideal plant to study floral color formation in ornamental shrubs. In our current work, HPLC analysis revealed that the principal pigments in tepals were the metabolite of flavonoids. All the tepals were containing quercetin, kaempferol 3‑O‑rutinoside and rutin while cyanidin‑3‑O‑glucoside and cyanidin‑3‑O‑rutinoside were only found in the in the red tepals. Moreover, we found the rutin as the principal component of all the pigments revealed. As well as in this study, a reference transcriptome library constructed from two varieties H29 and H64 flower. Further, 30 proteins of flavonoid biosynthesis pathway were identified in H29 flower using proteome analysis. Based on these dataset, the flavonoid biosynthesis pathway was also speculated. After quantitative analysis of gene expression, we found that ANS act as an on-off switch for the accumulation of red pigments and had positive correlations with various steps genes of the flavonoid pathway. This expression profiling demonstrates that no gene products compete for common substrates to redirect the metabolic flux in wintersweet. It is also demonstrated that high expression ofF3′Hwould provide sufficient content of the precursor, dihydroquercetin, for both flavonol and anthocyanin biosynthesis. The results help us to deepen and enrich the gene resource of color formation in wintersweet flower, and provide specific breeding strategies for increasing diversity of flower color.