MUTANTS OF SALMONELLA-TYPHIMURIUM THAT CANNOT SURVIVE WITHIN THE MACROPHAGE ARE AVIRULENT
MUTANTS OF SALMONELLA-TYPHIMURIUM THAT CANNOT SURVIVE WITHIN THE MACROPHAGE ARE AVIRULENT
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DOI：
10.1073/pnas.83.14.5189
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发表时间：
1986-07-01
影响因子：
11.1
通讯作者：
HEFFRON, F
中科院分区：
文献类型：
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作者：
FIELDS, PI;SWANSON, RV;HEFFRON, F
鼠伤寒沙门氏菌是一种兼性细胞内病原体，能在网状内皮系统的吞噬细胞内存活。为了确定细胞内生存的重要基因，在一株鼠伤寒沙门氏菌中分离到9516个独立的Tn10插入突变。通过使用巨噬细胞内存活的体外试验，已经鉴定出83个Tn10突变体具有降低的细胞内存活能力(被称为MS或巨噬细胞存活突变体)。所有的MS突变株在体内的毒力都低于亲本菌株，这表明对于沙门氏菌来说，在巨噬细胞内的生存对毒力是必不可少的。37个MS突变体已经根据其表型进行了表征，其中包括几个突变，这些突变使人对巨噬细胞的特定杀菌机制具有敏感性。
Salmonella typhimurium is a facultative intracellular pathogen capable of surviving within phagocytic cells of the reticuloendothelial system. To identify the genes important for intracellular survival, 9516 independent Tn10 insertional mutations were isolated in a virulent strain of S. typhimurium. By using an in vitro assay for survival within macrophages, 83 Tn10 mutants have been identified that have a diminished capacity for intracellular survival (designated MS or macrophage survival mutants). All of the MS mutants are less virulent than the parent strain in vivo, demonstrating that, for Salmonella, survival within the macrophage is essential for virulence. Thirty-seven of the MS mutants have been characterized as to their phenotype, including several mutations that confer sensitivity to specific microbiocidal mechanisms of the macrophage.