Genistein inhibits growth of estrogen-independent human breast cancer cells in culture but not in athymic mice.
Genistein inhibits growth of estrogen-independent human breast cancer cells in culture but not in athymic mice.
复制标题
DOI：
10.1093/jn/130.7.1665
复制
发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
R. Santell;R. Santell;N. Kieu;W. Helferich;W. Helferich
中科院分区：
文献类型：
--
作者：
R. Santell;R. Santell;N. Kieu;W. Helferich;W. Helferich
本研究旨在评估大豆异黄酮对雌激素非依赖性人乳腺癌细胞（MDA-MB-231）体外和体内增殖的影响。金雀异黄素（20 mcmol/L）在体外抑制细胞增殖约50%。40和80 mcmol/L染料木黄酮可阻断G2/M细胞周期进程。为了评估膳食染料木黄酮对体内肿瘤生长的影响，将染料木黄酮喂给接种MDA-MB-231细胞的雌性无胸腺小鼠。在实体瘤块形成后，以显示产生约1 mcmol/L的总血浆染料木黄酮浓度的剂量（750 mcg/g AIN-93 G饮食）给小鼠喂食染料木黄酮。此剂量的染料木黄酮对肿瘤生长无显著影响（P > 0.05）。然后进行研究以评估膳食染料木黄酮对初始肿瘤发展和生长的影响。在将细胞接种到小鼠中之前3天喂食染料木黄酮（750 mcg/g AIN-93 G饮食），不显著（P > 0.05）抑制肿瘤形成或生长。喂食该剂量膳食染料木黄酮（750 mcg/g AIN-93 G膳食）的小鼠中染料木黄酮的血浆浓度似乎不足以抑制肿瘤形成或生长。750 mcg/g AIN-93 G饮食中的膳食染料木黄酮不抑制肿瘤形成或生长。进行另外的研究以确定范围为0至6000 mcg/g AIN-93 G饮食的饮食剂量对血浆染料木黄酮浓度的影响。在6000 mcg/g AIN-93 G饲料中，血浆染料木黄酮浓度以剂量依赖性方式增加至7 mcmol/L。这些数据表明，虽然染料木黄酮在体外抑制癌细胞生长，但不太可能从膳食剂量的染料木黄酮中获得抑制体内癌细胞生长所需的血浆浓度。
The studies presented were conducted to assess the effect of the soy isoflavone genistein on proliferation of estrogen-independent human breast cancer cells (MDA-MB-231) in vitro and in vivo. Genistein (20 mcmol/L) inhibited cell proliferation in vitro by approximately 50%. Cell cycle progression was blocked in G(2)/M with 40 and 80 mcmol/L genistein. To evaluate the effect of dietary genistein on tumor growth in vivo, genistein was fed to female athymic mice inoculated with MDA-MB-231 cells. After solid tumor masses had formed, mice were fed genistein at a dose (750 mcg/g AIN-93G diet), shown to produce a total plasma genistein concentration of approximately 1 mcmol/L. This dose of genistein did not significantly (P > 0.05) alter tumor growth. Studies were then conducted to assess the effect of dietary genistein on initial tumor development and growth. Genistein (750 mcg/g AIN-93G diet), fed 3 d before cells were inoculated into mice, did not significantly (P > 0.05) inhibit tumor formation or growth. The plasma concentration of genistein in mice fed this dose of dietary genistein (750 mcg/g AIN-93G diet) does not appear sufficient to inhibit tumor formation or growth. Dietary genistein at 750 mcg/g AIN-93G diet does not inhibit tumor formation or growth. Additional studies were conducted to determine the effect of dietary dosages ranging from 0 to 6000 mcg/g AIN-93G diet on plasma genistein concentration. Plasma genistein concentration increased in a dose-dependent manner up to 7 mcmol/L at 6000 mcg/g AIN-93G diet. These data suggest that although genistein inhibits cancer cell growth in vitro, it is unlikely that the plasma concentration required to inhibit cancer cell growth in vivo can be achieved from a dietary dosage of genistein.