A triaxial probe for on-line proteolysis coupled with hydrogen/deuterium exchange-electrospray mass spectrometry.
A triaxial probe for on-line proteolysis coupled with hydrogen/deuterium exchange-electrospray mass spectrometry.
复制标题
用于在线蛋白水解与氢/氘交换电喷雾质谱联用的三轴探针。
DOI：
10.1016/j.jasms.2006.09.011
复制
发表时间：
2007
影响因子：
3.2
通讯作者：
Wetzel,Ronald
中科院分区：
文献类型：
--
作者：
Chen,Maolian;Cook,KelseyD;Kheterpal,Indu;Wetzel,Ronald
An on-line proteolysis system utilizing a triaxial electrospray probe was developed to aid localization of the hydrogen-bonding interaction sites in hydrogen/deuterium exchange-mass spectrometry (HDX-MS) studies of Aβ(1–40) fibrils. The probe allows delayed introduction of the organic solvent component needed for stable electrospray, thus enhancing hydrolysis performance relative to that of a coaxial probe. Effective on-line digestion was accomplished in ∼12 s. The probe should be of general utility for HDX-MS studies of amyloid fibrils and other protein aggregates.
