DEXTRAN SULFATE PREVENTS LTB4-INDUCED PERMEABILITY INCREASE, BUT NOT NEUTROPHIL EMIGRATION, IN THE HAMSTER-CHEEK POUCH
DEXTRAN SULFATE PREVENTS LTB4-INDUCED PERMEABILITY INCREASE, BUT NOT NEUTROPHIL EMIGRATION, IN THE HAMSTER-CHEEK POUCH
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DOI：
10.1016/0026-2862(89)90003-4
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发表时间：
1989-11-01
影响因子：
3.1
通讯作者：
ARFORS, KE
中科院分区：
文献类型：
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作者：
ROSENGREN, S;LEY, K;ARFORS, KE
已知白三烯 B4 (LTB4) 会在仓鼠颊囊中诱导中性粒细胞依赖性大分子渗漏。 LTB4 被灌注在颊囊上，进行活体显微镜检查；并对白细胞滚动和小静脉中的牢固粘附、中性粒细胞迁移和荧光葡聚糖渗漏时的大分子通透性进行定量。硫酸葡聚糖（MW 500,000；17.5 mg/kg 静脉推注）（但不带电的葡聚糖）可将 LTB4 诱导的大分子静脉渗漏减少 85%。组胺引起的渗漏与中性粒细胞无关，不受影响。硫酸葡聚糖对 LTB4 诱导的毛细血管后微静脉中的白细胞粘附没有影响，也对其随后迁移到周围组织没有影响。在非刺激条件下，硫酸葡聚糖显着抑制白细胞沿着集合小静脉壁滚动，但不抑制毛细血管后小静脉。因此，中性粒细胞诱导的大分子渗漏和中性粒细胞迁移似乎是独立的、可分离的事件。作为对本结果的解释，提出硫酸葡聚糖与中性粒细胞颗粒阳离子蛋白复合，从而防止中性粒细胞介导的通透性增加。
Leukotriene B4 (LTB4) is known to induce neutrophil-dependent macromolecular leakage in the hamster cheek pouch. LTB4 was superfused over cheek pouches dissected for intravital microscopy; and leukocyte rolling and firm adhesion in venules, neutrophil emigration, and macromolecular permeability as leakage of fluorescent dextran was quantified. Dextran sulfate (MW 500,000; 17.5 mg/kg iv bolus), but not uncharged dextran, reduced the LTB4-induced venular leakage of macromolecules by 85%. Histamine-induced leakage, which is neutrophil independent, was left unaffected. Dextran sulfate had no effect on leukocyte adhesion in postcapillary venules induced by LTB4, nor on their subsequent emigration to the surrounding tissue. Dextran sulfate significantly inhibited leukocyte rolling during nonstimulated conditions along the walls of collecting venules, but not postcapillary venules. Consequently, neutrophil-induced macromolecular leakage and neutrophil emigration appear to be independent, separable events. As an explanation of the present results it is proposed that the dextran sulfate complexes neutrophil granule cationic proteins, thus preventing neutrophil-mediated permeability increase.