Development of catheter-type optical oxygen sensor and applications to bioinstrumentation.
Development of catheter-type optical oxygen sensor and applications to bioinstrumentation.
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DOI：
10.1016/s0956-5663(03)00072-1
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发表时间：
2003-10
影响因子：
12.6
通讯作者：
K. Tsukada;S. Sakai;K. Hase;H. Minamitani
中科院分区：
文献类型：
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作者：
K. Tsukada;S. Sakai;K. Hase;H. Minamitani
研制了一种基于磷光寿命的导管式光学氧传感器，用于医学和动物实验。由于传感器探头应具有生物相容性和体内高透氧性，因此我们将重点放在了可接受的聚合物材料上，作为隐形眼镜探头的衬底。pd -卟啉被掺杂在硅基聚合物中，并被固定在插入导管的光纤边缘。探针的形状为直径600 μm，厚度100 μm，探针的氧渗透性高，Dk值为455。在精度评估中，氧传感器通过磷光寿命测量的po2值与氧电极作为校准数据测量的po2值之间存在良好的相关性。从150到0 mmHg的可逆默认值和从0到150 mmHg的可逆默认值达到90%所需的响应时间分别为15.43秒和7.52秒。此外，我们还研究了光学氧传感器的其他性能，如温度和pH依赖性、响应性和耐久性。在动物实验中，通过大鼠股动脉插入导管式氧传感器，在窒息状态下监测动脉氧压。该传感器在氧气浓度范围内有效，足以进行生物测量，并有望与留置针集成。
A catheter-type optical oxygen sensor based on phosphorescence lifetime was developed for medical and animal experimental use. Since the sensor probe should have biocompatibility and high oxygen permeability in vivo, we focused attention on acceptable polymer materials for contact lenses as the substrates of probes. Pd-porphyrin was doped in silicone-based polymer, and was fixed at the edge of an optical fiber inserted in a catheter tube. The shape of the probe was 600 μm in diameter and 100 μm in thickness, and the probe had high oxygen permeability of Dk value 455. In accuracy evaluation, there found an excellent correlation between the pO2values measured through phosphorescence lifetime using the oxygen sensors and those measured as the calibrating data using oxygen electrodes. The response time required to achieve 90% from reversible default value to be from 150 to 0 mmHg, and from 0 to 150 mmHg was 15.43 and 7.52 s, respectively. In addition, other properties such as temperature and pH dependency, response, and durability of our optical oxygen sensor were investigated. In animal experiments, the catheter-type oxygen sensor was inserted via the femoral artery of a rat, and arterial oxygen pressure was monitored under asphyxiation. The sensor was valid in the range of oxygen concentration sufficient for biometry, and expected to be integrated with an indwelling needle.