Expression of DII4 and CCL25 in Foxn1-negative epithelial cells in the post-natal thymus
Expression of DII4 and CCL25 in Foxn1-negative epithelial cells in the post-natal thymus
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DOI：
10.1093/intimm/dxl129
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发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
Amagai, Takashi
中科院分区：
文献类型：
--
作者：
Itoi, Manami;Tsukamoto, Noriyuki;Amagai, Takashi
Foxn1转录因子在胸腺器官的发育中是必不可少的。我们分析了Foxn1在胸腺上皮细胞中的表达对于Delta-like 4 （Dll4）和CCL25等功能分子的表达是否必要，以及这些分子表达的维持是否依赖于Foxn1转录因子。我们发现几乎所有的早期胸腺上皮细胞都表达Foxn1，而Dll4和CCL25在Foxn1阳性的上皮细胞中表达有限。该结果与先前的报道一致，认为Foxn1对于上皮细胞分化是不可或缺的，这使得这些细胞能够诱导CCL25的表达（Bleul, c.c.和Boehm， T. 2000）。趋化因子在发育中的胸腺中定义不同的微环境。欧元。J.免疫学，30:3371)，Dll1和Dll4 (Tsukamoto， N., Itoi， M., Nishikawa， M. and Amagai， T. 2005。在裸胸腺标本中缺乏δ 1和δ 4的表达。细胞。Immunol 234:77)。另一方面，在大量出生后胸腺上皮细胞中未检测到Foxn1的表达。foxn1阳性和阴性的上皮细胞似乎都表达Dll4和CCL25。因此，Dll4和CCL25在出生后胸腺中的表达不依赖于Foxn1转录因子。这些结果表明，在出生后的胸腺中，上皮细胞可以在没有Foxn1转录因子的帮助下维持这些功能分子的表达。
Foxn1 transcription factor is known to be essential for development of the thymic organ. We analyzed whether Foxn1 expression in thymic epithelial cells is necessary for the expression of functional molecules such as Delta-like 4 (Dll4) and CCL25, and whether maintenance of these molecular expressions depends on the Foxn1 transcription factor. We show that almost all thymic epithelial cells in the early thymus anlagen express Foxn1, and Dll4 and CCL25 are limitedly expressed in Foxn1-positive epithelial cells. The results are consistent with previous reports suggesting the indispensability of Foxn1 for epithelial cell differentiation which enables these cells to induce the expressions of CCL25 (Bleul, C. C. and Boehm, T. 2000. Chemokines define distinct microenvironments in the developing thymus. Eur. J. Immunol. 30:3371), Dll1 and Dll4 (Tsukamoto, N., Itoi, M., Nishikawa, M. and Amagai, T. 2005. Lack of Delta like 1 and 4 expressions in nude thymus anlages. Cell. Immunol. 234:77). On the other hand, the expression of Foxn1 was not detectable in a large number of post-natal thymic epithelial cells. Both Foxn1-positive and -negative epithelial cells seem to express Dll4 and CCL25. Therefore, the expressions of Dll4 and CCL25 are independent of Foxn1 transcription factor in the post-natal thymus. These results indicate that in the post-natal thymus, epithelial cells may maintain the expressions of those functional molecules without the aid of Foxn1 transcription factor.