MONOCYTE CHEMOTACTIC PROTEINS MCP-1, MCP-2, AND MCP-3 ARE MAJOR ATTRACTANTS FOR HUMAN CD4(+) AND CD8(+) T-LYMPHOCYTES
MONOCYTE CHEMOTACTIC PROTEINS MCP-1, MCP-2, AND MCP-3 ARE MAJOR ATTRACTANTS FOR HUMAN CD4(+) AND CD8(+) T-LYMPHOCYTES
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DOI：
10.1096/fasebj.8.13.7926371
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发表时间：
1994-10-01
期刊：
影响因子：
4.8
通讯作者：
MOSER, B
中科院分区：
文献类型：
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作者：
LOETSCHER, P;SEITZ, M;MOSER, B
使用克隆的人CD 4（+）和CD 8（+）T细胞研究了淋巴细胞对六种CC趋化因子MCP-1、MCP-2、MCP-3、MIP-1 α、MIP-1 β和RANTES的反应。所有测试的CC趋化因子诱导两种类型的淋巴细胞的迁移，而用作对照的两种CXC趋化因子IL-8和IP-10是无活性的。单核细胞趋化蛋白（MCP-1、MCP-2和MCP-3）显示出典型的双峰浓度依赖性，并且比MIP-1 α、MIP-1 β或RANTES有效得多。所有CC趋化因子也诱导细胞溶质游离Ca 2+在任一类型的T细胞中的快速和短暂的上升。百日咳杆菌毒素治疗阻止了这种上升，表明G蛋白偶联受体参与了信号传导。这在MCP-1和MCP-3中最明显，这与这些趋化因子作为化学引诱剂的功效一致。对MCP-2、MIP-1 α、MIP-1 β和RANTES的反应较弱，并且在用IL-8或IP-10刺激时未获得变化。新鲜分离的人血淋巴细胞也进行了测试，但既没有迁移也没有观察到Ca 2+的变化。在CD 4（+）和CD 8（+）细胞上发现少量的MCP-1高亲和力受体（< 900个/细胞，K-d
The responses of lymphocytes to six CC chemokines-MCP-1, MCP-2, MCP-3, MIP-1 alpha, MIP-1 beta, and RANTES-were studied using cloned human CD4(+) and CD8(+) T cells. All CC chemokines tested induced migration of both types of lymphocytes, whereas two CXC chemokines used as controls, IL-8 and IP-10, were inactive. The monocyte chemotactic proteins (MCP-1, MCP-2, and MCP-3) showed a typically bimodal concentration dependence, and were considerably more effective than MIP-1 alpha, MIP-1 beta, or RANTES. All CC chemokines also induced a rapid and transient rise in cytosolic free Ca2+ in either type of T cell. The rise was prevented by Bordetella pertussis toxin treatment, indicating that G-protein-coupled receptors are involved in signaling. It was most pronounced with MCP-1 and MCP-3, which is in agreement with the efficacy of these chemokines as chemoattractants. The responses to MCP-2, MIP-1 alpha, MIP-1 beta, and RANTES were weaker, and no changes were obtained on stimulation with IL-8 or IP-10. Freshly isolated human blood lymphocytes were also tested, but neither migration nor Ca2+ changes were observed. Low numbers of high-affinity receptors for MCP-1 were found on CD4(+) and CD8(+) cells (< 900 per cell, K-d