DEVELOPING CORRELATIONS BETWEEN FIBRINOGEN ADSORPTION AND SURFACE-PROPERTIES USING MULTIVARIATE-STATISTICS
DEVELOPING CORRELATIONS BETWEEN FIBRINOGEN ADSORPTION AND SURFACE-PROPERTIES USING MULTIVARIATE-STATISTICS
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DOI：
10.1002/jbm.820281002
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发表时间：
1994-10-01
期刊：
影响因子：
--
通讯作者：
RATNER, BD
中科院分区：
文献类型：
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作者：
PEREZLUNA, VH;HORBETT, TA;RATNER, BD
基于偏最小二乘算法（PLS）的多变量模型的构建，以建立一个常见的聚合物材料的表面性质和纤维蛋白原的吸附量和保留从复杂的混合物之间的相关性。通过静态二次离子质谱（SIMS），电子能谱化学分析（ESCA），并通过接触角测量的几种液体对这些材料进行表面表征。从缓冲液中的1%血浆溶液中吸附I-125-纤维蛋白原，并测定2小时后的吸附量。在缓冲液中停留5天后，用1%的表面活性剂十二烷基硫酸钠（SDS）溶液洗脱吸附的纤维蛋白原。洗脱后保留在表面上的纤维蛋白原的百分比称为纤维蛋白原保留。纤维蛋白原的吸附量或纤维蛋白原保留的表面性质和量之间的相关性建立。这些模型还显示了与这些表面上的蛋白质行为相关的最重要的变量。(C)John Wiley & Sons，Inc.
A multivariate model based on the partial least squares algorithm (PLS) was constructed in order to establish a correlation between the surface properties of common polymeric materials and the amount and retention of fibrinogen adsorbed from a complex mixture. Surface characterization was performed by means of static secondary ion mass spectroscopy (SIMS), electron spectroscopy for chemical analysis (ESCA), and by contact angle measurements of several liquids on those materials. I-125-fibrinogen was adsorbed from a 1% plasma solution in buffer and the amount adsorbed after 2 h was determined. After 5 days of residence time in buffer, the adsorbed fibrinogen was eluted with a 1% solution of the surfactant sodium dodecyl sulfate (SDS). The percent of fibrinogen that remained on the surfaces after elution is referred to as fibrinogen retention. Correlations between surface properties and the amounts of fibrinogen adsorbed or fibrinogen retention were established. These models also show the most important variables that are related to the protein behavior on these surfaces. (C) 1994 John Wiley & Sons, Inc.