The molecular basis for water taste in Drosophila.
The molecular basis for water taste in Drosophila.
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水的检测和水分摄入的调节对于动物维持适当的渗透平衡至关重要。果蝇和其他昆虫具有味觉感觉神经元，它们介导对外部水源的识别，但对于水味检测的潜在分子机制知之甚少。在此，我们鉴定出退化蛋白/上皮钠通道家族的一个成员ppk28，它是一种渗透敏感离子通道，介导对水的细胞和行为反应。我们利用分子、细胞、钙成像和电生理方法表明，ppk28在水感应神经元中表达，ppk28缺失会消除水敏感性。此外，ppk28的异位表达使果蝇中感受苦味的味觉神经元对水敏感，并且当在异源细胞中表达时对低渗溶液敏感。这些研究将一种渗透敏感离子通道与水味检测和饮水行为联系起来，为研究其他动物水检测的分子基础提供了框架。
The detection of water and the regulation of water intake are essential for animals to maintain proper osmotic homeostasis. Drosophila and other insects have gustatory sensory neurons that mediate the recognition of external water sources, but little is known about the underlying molecular mechanism for water taste detection. Here, we identify a member of the Degenerin/Epithelial Sodium Channel family, ppk28, as an osmosensitive ion channel that mediates the cellular and behavioral response to water. We use molecular, cellular, calcium imaging and electrophysiological approaches to show that ppk28 is expressed in water-sensing neurons and loss of ppk28 abolishes water sensitivity. Moreover, ectopic expression of ppk28 confers water sensitivity to bitter-sensing gustatory neurons in the fly and sensitivity to hypo-osmotic solutions when expressed in heterologous cells. These studies link an osmosensitive ion channel to water taste detection and drinking behavior, providing the framework for examining the molecular basis for water detection in other animals.
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影响因子：
20.1
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