Spatially restricted expression of a member of a new family of murine Distal-less homeobox genes in the developing forebrain.
Spatially restricted expression of a member of a new family of murine Distal-less homeobox genes in the developing forebrain.
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小鼠远端无同源盒基因新家族成员在发育前脑中的空间受限表达。
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
K. Mahon
中科院分区：
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作者：
G. Robinson;S. Wray;K. Mahon
果蝇的同源异型盒基因在胚胎模式形成中起关键作用，它们在脊椎动物中的对应基因可能起类似的发育作用。利用聚合酶链反应技术，我们已经确定了四个小鼠同源的果蝇远侧同源异型盒基因，在妊娠中期胚胎中表达。这些基因编码的同源结构域与其他已知的同源结构域序列有很大的不同，代表了一个新的脊椎动物同源盒基因家族。我们分离出这些基因之一（Dlx-2）的cDNA，并通过原位杂交研究其表达，从出生后8.5天（PC），直到出生后第1天。Dlx-2在腹侧前脑中显示出有限的表达模式，从嗅球延伸到腹侧间脑。这一表达领域可能描绘了前脑内个体发育学定义的细分。小鼠Distal-less基因是第一个描述的同源框基因，其在中枢神经系统中的表达仅限于前脑。因此，远端少的基因可能会造成丢失的位置线索不提供由先前确定的脊椎动物同源框基因。
The homeobox genes of Drosophila perform key functions in embryonic pattern formation, and their vertebrate counterparts may play similar developmental roles. Using polymerase chain reaction technology, we have identified four murine homologs of the Drosophila Distal-less homeobox gene that are expressed in midgestation embryos. The homeodomains encoded by these genes vary considerably from other known homeodomain sequences and represent a new family of vertebrate homeobox genes. We isolated a cDNA for one of these genes (Dlx-2) and studied its expression by in situ hybridization from 8.5 days postcoitum (pc) until postnatal day 1. Dlx-2 shows a restricted pattern of expression in the ventral forebrain, extending from the olfactory bulb to the ventral diencephalon. This domain of expression may delineate an ontogenetically defined subdivision within the forebrain. The murine Distal-less genes are the first homeobox genes described whose expression in the central nervous system is exclusively restricted to the forebrain. Thus, the Distal-less genes may contribute missing positional cues not provided by the previously identified vertebrate homeobox genes.