Production and characterization of murine single chain Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system
Production and characterization of murine single chain Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system
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DOI：
10.1080/0954010021000096373
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发表时间：
2002-12-01
影响因子：
3
通讯作者：
O'Kennedy, R
中科院分区：
文献类型：
--
作者：
Daly, SJ;Dillon, PP;O'Kennedy, R
描述了抗黄曲霉毒素B-1（AF B（1））（一种强效真菌毒素和致癌物）的重组抗体的生产和表征。然后将产生的抗体片段应用于基于表面等离子体共振的生物传感器（BIAcore）中，该生物传感器“实时”测量生物分子相互作用。从已建立的噬菌体展示系统产生抗黄曲霉毒素B-1的单链Fv（scFv）抗体，所述噬菌体展示系统并入一系列不同的质粒用于有效的scFv表达。scFv用于竞争性ELISA的开发，并且还用于基于表面等离子体共振（SPR）的抑制免疫测定的开发。它们被发现适用于检测AFB（1），在这种形式下，该测定法灵敏且可重复。
The production and characterization of recombinant antibodies to aflatoxin B-1 (AFB(1) ), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B-1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB(1) , in this format, with the assays being sensitive and reproducible.