A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition
A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition
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DOI：
10.1007/s003350020019
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发表时间：
2001-08-01
期刊：
影响因子：
2.5
通讯作者：
Rothschild, MF
中科院分区：
文献类型：
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作者：
Malek, M;Dekkers, JCM;Rothschild, MF
分子遗传标记可用于鉴定含有控制家畜肉质和肌肉组成性状的数量性状基因座（QTL）的染色体区域。为了在猪中研究这一点，从两个伯克希尔大公猪和九个约克郡大母猪之间的杂交产生了一个资源家族。从65个F-1亲本的杂交中共产生525个F-2后代。28个肉质性状（滴水损失，持水力，硬度，颜色，大理石花纹，百分之胆固醇，最终pH值，纤维类型，和几个感官面板和冷却性状）的表型数据收集的F-2动物。对动物进行了覆盖整个基因组的125个微卫星标记的基因分型。QTL检测采用最小二乘回归区间作图法。通过排列检验确定显著性阈值。在5%染色体水平上检测到60个肉质性状QTL，分别位于第1、2、4、5、6、7、8、10、11、12、13、14、15、17、18和X染色体上，其中9个和1个QTL在5%和1%基因组水平上显著高于第1、5、12、15和17染色体。分别
Molecular genetic markers can be used to identify chromosomal regions that contain quantitative trait loci (QTL) that control meat quality and muscle composition traits in farm animals. To study this in pigs, a resource family was generated from a cross between two Berkshire grand sires and nine Yorkshire grand dams. A total of 525 F-2 progeny from 65 matings of F-1 parents were produced. Phenotypic data on 28 meat quality traits (drip loss, water holding capacity, firmness, color, marbling, per centage cholesterol, ultimate pH, fiber type, and several sensory panel and cooling traits) were collected on the F-2 animals. Animals were genotyped for 125 microsatellite markers covering the entire genome. Least squares regression interval mapping was used for QTL detection. Significance thresholds were determined by permutation tests. A total of 60 QTL were detected at the 5% chromosome level for meat qualify traits, on Chrs 1, 2, 4, 5, 6, 7, 8, 10, 1 1, 12, 13, 14, 15, 17, 18, and X, of which 9 and 1 QTL were significant at the 5% and 1% genome-wise levels ton Chrs 1,5, 12, 15, and 17), respectively.