The number distribution of complex shear modulus of single cells measured by atomic force microscopy
The number distribution of complex shear modulus of single cells measured by atomic force microscopy
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DOI：
10.1016/j.ultramic.2009.03.008
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发表时间：
2009-07-01
期刊：
影响因子：
2.2
通讯作者：
Okajima, Takaharu
中科院分区：
文献类型：
--
作者：
Hiratsuka, Shinichiro;Mizutani, Yusuke;Okajima, Takaharu
采用原子力显微镜（AFM）与微阵列技术相结合的方法研究了大量小鼠成纤维细胞NIH3T3细胞（n = 130）的粘弹性特性。在实验中，将细胞排列培养在微阵列衬底的孔中，并采用力调制模式实验测量0.5-200 Hz频率范围内单个细胞的复合剪切模量G*。细胞G*的频率依赖性表现为幂律行为，并且在平面基质上培养的几种细胞类型中观察到类似的频率依赖性。这表明在微阵列孔中培养的NIH3T3细胞与在平面基质上培养的细胞具有类似的结构组织。G*的存储模量和损耗模量的数量分布都符合对数正态分布函数，而幂律结构阻尼模型估计的幂律指数则符合正态分布函数。这些结果表明，将AFM与微阵列技术相结合是一种不需要对细胞表面进行修饰就能研究活细胞流变特性统计的合适方法。(c) 2009 Elsevier B.V.版权所有
The viscoelastic properties of a large number of mouse fibroblast NIH3T3 cells (n similar or equal to 130) were investigated by combining atomic force microscopy (AFM) with a microarray technique. In the experiments, the cells were arranged and cultured in the wells of a microarray substrate, and a force modulation mode experiment was used to measure the complex shear modulus, G*, of individual cells in a frequency range 0.5-200 Hz. The frequency dependence of G* of the cells exhibited a power-law behavior and similar frequency dependencies have been observed in several cell types cultured on flat substrates. This indicated that the NIH3T3 cells cultured in the wells of a microarray have analogous structural organization to those cells cultured on flat substrates. The number distribution of both the storage and loss moduli of G* fitted well to a log-normal distribution function, whereas the power-law exponent estimated by a power-law structural damping model showed a normal distribution function. These results showed that combining AFM with a microarray technique was a suitable approach for investigating the statistics of rheological properties of living cells without the requirement of cell surface modification. (c) 2009 Elsevier B.V. All rights reserved.