Genomic and proteomic analysis of the myeloid differentiation program
Genomic and proteomic analysis of the myeloid differentiation program
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DOI：
10.1182/blood.v98.3.513
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发表时间：
2001-08-01
期刊：
影响因子：
20.3
通讯作者：
Weissman, SM
中科院分区：
文献类型：
--
作者：
Lian, Z;Wang, L;Weissman, SM
虽然成熟中性粒细胞是哺乳动物细胞类型中较好表征的细胞类型之一，但在分子水平上对髓样分化的机制还不完全了解。一种小鼠早幼粒细胞系（MPRO），来源于鼠骨髓细胞并被显性负性视黄酸受体阻止发育，在10 μ M视黄酸存在下形态学分化为成熟的嗜中性粒细胞。一个广泛的目录是准备在形态成熟过程中发生的基因表达变化。为此，3 '端差异显示，寡核苷酸，芯片阵列杂交，和二维蛋白电泳。大量的基因，其mRNA水平的MPRO细胞的分化过程中进行了调节。结果表明，参与的几个转录调控因子以前没有牵连在这个过程中，但他们也强调了事件的重要性，而不是新的转录因子的生产。在mRNA和蛋白质水平上比较基因表达模式，并研究2个参数之间的相关性。
Although the mature neutrophil Is one of the better characterized mammalian cell types, the mechanisms of myeloid differentiation are Incompletely understood at the molecular level. A mouse promyelocytic cell line (MPRO), derived from murine bone marrow cells and arrested developmentally by a dominant-negative retinoic acid receptor, morphologically differentiates to mature neutrophils In the presence of 10 muM retinoic acid. An extensive catalog was prepared of the gene expression changes that occur during morphologic maturation. To do this, 3 ' -end differential display, oligonucleotide, chip array hybridization, and 2-dimensional protein electrophoresis were used. A large number of genes whose mRNA levels are modulated during differentiation of MPRO cells were identified. The results suggest the involvement of several transcription regulatory factors not previously implicated in this process, but they also emphasize the importance of events other than the production of new transcription factors. Furthermore, gene expression patterns were compared at the level of mRNA and protein, and the correlation between 2 parameters was studied.