The study of rheological effects on vascular endothelial cells in culture.
The study of rheological effects on vascular endothelial cells in culture.
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DOI：
10.3233/bir-1989-26218
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发表时间：
1989
期刊：
影响因子：
1.1
通讯作者：
M. Levesque;R. Nerem
中科院分区：
文献类型：
--
作者：
M. Levesque;R. Nerem
许多细胞培养研究报道了剪切应力对血管内皮细胞的影响。虽然通过这些研究已经了解了内皮细胞的水动力环境对其结构和功能的影响，但报告表明在方法上存在显着差异。使用单元格形状作为可能由不同方法导致的差异的指标，对选定变量的影响进行了调查。结果表明，不仅剪切应力水平和暴露时间等变量很重要，而且基材、介质组成、电池本身的特性以及流动的性质（例如是稳态还是脉动）也很重要。
A number of cell culture studies have been reported on the influence of shear stress on vascular endothelial cells. Although through such studies much has been learned about the effect of an endothelial cell's hydrodynamic environment on its structure and function, the reports indicate significant differences in methodology. Using cell shape as an indicator of differences that might result from differing methodologies, an investigation of the influence of selected variables has been carried out. The results presented indicate that not only are such variables as the level of shear stress and the duration of exposure important, but also substrate, media composition, characteristics of the cell itself, and the nature of the flow, e.g. whether it is steady state or pulsatile.