The class A chitin synthase gene of Spodoptera exigua:: Molecular cloning and expression patterns
The class A chitin synthase gene of Spodoptera exigua:: Molecular cloning and expression patterns
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DOI：
10.1016/j.ibmb.2007.01.006
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发表时间：
2007-05-01
影响因子：
3.8
通讯作者：
Zhang, Wenqing
中科院分区：
文献类型：
--
作者：
Chen, Xiaofei;Yang, Xing;Zhang, Wenqing
几丁质合成酶（CHS）是其他组织角质层和角质层内层形成几丁质所必需的重要酶促成分。本研究从甜菜夜蛾Spodoptera exigua (Hubner) （Se）中鉴定了一个新的CHS基因。同源。对S. exigua CHS （SeCHS）与其他相关蛋白的比对和系统发育分析表明，SeCHS属于A类CHS家族（SeCHSA）。Northern blot分析显示，SeCHSA优先在表皮和气管中转录。进一步的研究表明，SeCHSA mRNA在各幼虫的早期和晚期都有高表达，并且在蛹期持续高表达。使用CHS特异性抗体，SeCHS进一步定位于被膜和气管细胞的下表皮细胞，但不在脂肪体或马尔比氏小管中。这些数据表明，SeCHS在香瓜角质层的形成和发育中起着重要的作用。(C) 2007 Elsevier Ltd.版权所有。
Chitin synthase (CHS) is an important enzymatic component required for chitin formation in the cuticles and cuticular linings of other tissues. In the present study, a new CHS gene was characterized from the beet armyworm Spodoptera exigua (Hubner) (Se). Homologous. alignment and phylogenetic analysis of S. exigua CHS (SeCHS) with other related proteins suggest that SeCHS belongs to the class A CHS family (SeCHSA). Northern blot analysis revealed that SeCHSA is transcribed preferentially in the cuticle and tracheae. Further investigation indicated that SeCHSA mRNA is highly expressed in the early and late stages of each larval instar, and consistently expressed in high level during the pupal stage. Using antibody specific for CHS, SeCHS was further localized in the underlying epidermal cells of the integument and tracheal cells, but not in the fat body or Malpighian tubules. These data suggest that SeCHS plays an important role in cuticle formation and development of S. exigua. (C) 2007 Elsevier Ltd. All rights reserved.