Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b.
Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b.
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DOI：
10.1016/j.ygeno.2003.09.018
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发表时间：
2004-04
期刊：
影响因子：
4.4
通讯作者：
M. Onishi;T. Yasunaga;Hiromitsu Tanaka;Y. Nishimune;M. Nozaki
中科院分区：
文献类型：
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作者：
M. Onishi;T. Yasunaga;Hiromitsu Tanaka;Y. Nishimune;M. Nozaki
OXCT/SCOT是许多肝外器官线粒体酮解速率的决定酶。两个睾丸同种异构体Oxct2a和Oxct2b是高度同源的，在小鼠的单倍体精子中特异性表达。在这篇报道中，我们分析了Oxct2a和Oxct2b的结构和进化。两个Oxct2都是单拷贝无内含子基因，其核苷酸序列与Oxct保守，表明这些基因是由Oxct产生的转座子。在两个Oxct2中都发现了一个CpG岛。Oxct2a和Oxct2b位于Bmp8a和Bmp8b的第三个内含子中，位于4号染色体上一个240-kb的尾尾定向区域内。这一结构特征在人类1p34.3的一个同工区也被保守。小鼠和人类之间的结构相似性表明，这两组基因是由片段基因复制产生的，这发生在灵长类动物和啮齿动物分裂之前。点阵和系统发育树分析表明，两个基因座之间的染色体内基因转换在每个物种中都独立发生了多轮。
OXCT/SCOT is the rate-determining enzyme in ketolysis in mitochondria of many extrahepatic organs. Two testicular isoforms, Oxct2a and Oxct2b, are highly homologous and specifically expressed in haploid spermatids of the mouse. In this report, we analyzed the structure and evolution of Oxct2a and Oxct2b. Both Oxct2's are single-copy intronless genes, of which nucleotide sequences are conserved with Oxct, indicating that these genes are transposons generated from Oxct. A CpG island was found within both Oxct2's. Oxct2a and Oxct2b are located in the third introns of Bmp8a and Bmp8b, and they are positioned within a 240-kb region in a tail-to-tail orientation on chromosome 4. This structural feature was also conserved in a syntenic region of human 1p34.3. Structural similarity between mice and humans indicated that these two sets of genes were generated by a segmental gene duplication, which occurred before the primate–rodent split. Dot matrix and phylogenetic tree analyses demonstrated that multiple rounds of intrachromosomal gene conversion between the two loci occurred in each species independently.