INDUCTION OF MEMBRANE RUFFLING AND FLUID-PHASE PINOCYTOSIS IN QUIESCENT FIBROBLASTS BY RAS PROTEINS
INDUCTION OF MEMBRANE RUFFLING AND FLUID-PHASE PINOCYTOSIS IN QUIESCENT FIBROBLASTS BY RAS PROTEINS
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DOI：
10.1126/science.3090687
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发表时间：
1986-09-05
期刊：
影响因子：
56.9
通讯作者：
FERAMISCO, JR
中科院分区：
文献类型：
--
作者：
BARSAGI, D;FERAMISCO, JR
Ras癌基因的表达被认为是某些类型人类癌症的始发事件之一。为了确定ras蛋白直接触发的细胞活动，研究了将人H-ras蛋白显微注射到静止的大鼠胚胎成纤维细胞中的早期后果。在注射后30分钟至1小时内，细胞表面皱纹和液相吞饮显著增加。H-ras蛋白的原癌和致癌两种形式都诱导了膜皱缩和胞饮作用的快速增强。致癌蛋白产生的影响在注射后持续超过15小时，而原致癌蛋白的影响是短暂的，仅限于注射后3小时的间隔。Ras癌基因蛋白对皱褶和吞饮作用的刺激作用依赖于注射的蛋白量，并伴随着对磷脂酶A2活性的明显刺激。Ras蛋白诱导的细胞膜活性的这些快速变化可能是ras蛋白作用机制中的主要事件。
Expressin of the ras oncogene is thought to be one of the contributing events in the initiation of certain types of human cancer. To determine the cellular activities that are directly triggered by ras proteins, the early consequences of microinjection of the human H-ras proteins into quiescent rat embryo fibroblasts were investigated. Within 30 minutes to 1 hour after injection, cells show a marked increase in surface ruffles and fluid-phase pinocytosis. The rapid enhancement of membrane ruffling and pinocytosis is induced by both the proto-oncogenic and the oncogenic forms of the H-ras protein. The effects produced by the oncogenic protein persist for more than 15 hours after injection, whereas the effects of the proto-oncogenic protein are short-lived, being restricted to a 3-hour interval after injection. The stimulatory effect of the ras oncogene protein on ruffling and pinocytosis is dependent on the amount of injected protein and is accompanied by an apparent stimulation of phospholipase A2 activity. These rapid changes in cell membrane activities induced by ras proteins may represent primary events in the mechanism of action of ras proteins.