Effect of environmental temperature on naphthalene metabolism by Juvenile Starry flounder (Platichthys stellatus)
Effect of environmental temperature on naphthalene metabolism by Juvenile Starry flounder (Platichthys stellatus)
复制标题
环境温度对星牙鲽幼鱼萘代谢的影响（Platichthys stellatus）
DOI：
10.1007/bf01055622
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发表时间：
1981
影响因子：
4
通讯作者：
W. Reichert
中科院分区：
文献类型：
--
作者：
U. Varanasi;D. Gmur;W. Reichert
维持在4°或12°C的星鲽幼鱼（Platichthys stellatus）被强制喂食3h -1-萘。在开始暴露24小时后，4°C条件下星形比目鱼组织中的萘浓度显著（p < 0.05）高于12°C条件下星形比目鱼组织中的萘浓度（2 ~ 15倍）。降低水温对萘保留率的影响在1周后更为显著。此时，4℃鱼的肌肉和肝脏的萘含量分别是12℃鱼的肌肉和肝脏的26倍和34倍。在萘暴露后24或168小时，大多数组织的总代谢物浓度在较低温度下并没有明显升高。代谢产物的薄层色谱分离表明，在24小时时，无论温度如何，1,2-二氢-1,2-二羟基萘（二氢二醇）是肝脏（提取代谢物的40 - 50%）和肌肉（提取代谢物的~ -80%）的主要成分。胆汁主要含有缀合物（如葡萄糖醛酸酯），在酶解作用下产生二氢二醇作为主要代谢物。24 ~ 168小时，各代谢物类别的浓度与总代谢物浓度没有直接变化。因此，在168小时时，4°C与12°C下鱼肝脏总代谢物浓度之比为1.6，而二氢二醇、硫酸盐/葡萄糖苷缀合物和葡萄糖醛酸缀合物的比例分别为4.5、0.6和3.8。水温降低，母体烃及其代谢物的组织浓度升高。然而，增加的幅度取决于化合物、组织和暴露开始后的时间。研究结果强调了在评估环境参数对外源毒性的影响时，确定鱼类组织中单个代谢物和母体碳氢化合物浓度的重要性。
Juvenile starry flounder (Platichthys stellatus) maintained at 4° or 12°C were forced-fed3H-1-naphthalene. At 24 hr, after the initiation of exposure, significantly (p < 0.05) higher concentrations (2 to 15 times) of naphthalene were present in tissues of starry flounder at 4°C than those present in fish held at 12°C. The influence of lowering of water temperature on naphthalene retention was even more marked after one week. At this time, muscle and liver of fish at 4°C contained 26 and 34 times, respectively, more naphthalene than did muscle and liver of fish at 12°C. Concentrations of total metabolites, in most tissues were not substantially higher at the lower temperature either 24 or 168 hr after the naphthalene-exposure.Thin-layer Chromatographic separation of the metabolites revealed that at 24 hr, 1,2-dihydro-1,2-dihydroxynaphthalene (dihydrodiol) was the major component in liver (40 to 50% of extracted metabolites) and muscle (∼-80% of extracted metabolites) regardless of the temperature. Bile contained, primarily, conjugates (e.g., glucuronides), which yielded the dihydrodiol as the principal metabolite on enzymatic hydrolysis. From 24 to 168 hr, the concentrations of each metabolite class did not vary directly with the concentrations of total metabolites. Accordingly, at 168 hr, the ratio of total metabolite concentrations in liver of fish at 4°C compared to 12°C was 1.6, whereas the ratios for the dihydrodiol, sulfate/glucoside conjugates and glucuronide conjugates were 4.5, 0.6 and 3.8 respectively.Generally, lowered water temperature increased tissue concentrations of the parent hydrocarbon and its metabolites. However, the magnitude of the increase was dependent upon the compound, the tissue, and the time after the initiation of the exposure. The results emphasize the importance of determining concentrations of individual metabolites together with parent hydrocarbons in tissues of fish when assessing effects of environmental parameters on xenobiotic toxicity.