Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages.
Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages.
复制标题
使用来自九个新旧世界谱系的结构蛋白检查人类抗体对新兴 Mayaro 病毒和共循环甲病毒感染的反应。
DOI：
10.1128/msphere.00003-18
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发表时间：
2018
期刊：
影响因子：
4.8
通讯作者：
Ulrich,RobertG
中科院分区：
文献类型：
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作者：
Smith,JessicaL;Pugh,ChristineL;Cisney,EmilyD;Keasey,SarahL;Guevara,Carolina;Ampuero,JuliaS;Comach,Guillermo;Gomez,Doris;Ochoa-Diaz,Margarita;Hontz,RobertD;Ulrich,RobertG
马雅罗病毒（MAYV）、委内瑞拉马脑炎病毒（VEEV）和基孔肯雅病毒（CHIKV）是在南美洲共同传播的媒介传播的甲病毒。这些病毒引起的人类感染经常被误诊或漏诊，特别是在登革热病毒高流行地区。疾病可发展为衰弱性关节痛（MAYV， CHIKV）、脑炎（VEEV）和死亡。很少有标准化的血清学检测方法用于检测特定的人甲病毒感染，抗原交叉反应性可能存在问题。因此，有助于特异性检测多种甲病毒感染的血清学平台将极大地扩大对这些新发感染的疾病监测。在这项研究中，使用重组衣壳、包膜蛋白1 （E1）和E2组装的蛋白质微阵列检测了南美经PCR和/或分离证实感染MAYV、VEEV和CHIKV的患者的血清样本。值得注意的是，仅在MAYV感染中观察到E1的特异性抗体识别，而E2被所有研究的甲病毒感染的抗体特异性靶向，只有在chikv感染患者的血清样本中才有对o 'nyong-nyong病毒E2的交叉反应。我们的研究结果表明，甲病毒结构蛋白微阵列可以区分由MAYV、VEEV和CHIKV引起的感染，并且这种多路血清学平台可用于高通量疾病监测。重要意义马亚罗病毒、基孔肯雅病毒和委内瑞拉马脑炎病毒是密切相关的甲病毒，通过蚊子传播，导致产生类似流感样症状或更严重疾病的疾病。此外，甲病毒感染的症状可能与登革热或寨卡病相似，导致病例少报和潜在的误诊。可用于在同一试验中检测对多种甲病毒的抗体反应的新方法将极大地有助于疾病监测工作。然而，病毒之间可能存在的抗体交叉反应会降低实验室结果的质量。我们的研究结果表明，通过选择重组蛋白抗原，可以在同一试验中特异性地定量抗体对多种甲型病毒的反应，并进一步表明Mayaro病毒感染对病毒包膜蛋白产生独特的反应。
Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), and chikungunya virus (CHIKV) are vector-borne alphaviruses that cocirculate in South America. Human infections by these viruses are frequently underdiagnosed or misdiagnosed, especially in areas with high dengue virus endemicity. Disease may progress to debilitating arthralgia (MAYV, CHIKV), encephalitis (VEEV), and death. Few standardized serological assays exist for specific human alphavirus infection detection, and antigen cross-reactivity can be problematic. Therefore, serological platforms that aid in the specific detection of multiple alphavirus infections will greatly expand disease surveillance for these emerging infections. In this study, serum samples from South American patients with PCR- and/or isolation-confirmed infections caused by MAYV, VEEV, and CHIKV were examined by using a protein microarray assembled with recombinant capsid, envelope protein 1 (E1), and E2 from nine New and Old World alphaviruses. Notably, specific antibody recognition of E1 was observed only with MAYV infections, whereas E2 was specifically targeted by antibodies from all of the alphavirus infections investigated, with evidence of cross-reactivity to E2 of o’nyong-nyong virus only in CHIKV-infected patient serum samples. Our findings suggest that alphavirus structural protein microarrays can distinguish infections caused by MAYV, VEEV, and CHIKV and that this multiplexed serological platform could be useful for high-throughput disease surveillance.IMPORTANCEMayaro, chikungunya, and Venezuelan equine encephalitis viruses are closely related alphaviruses that are spread by mosquitos, causing diseases that produce similar influenza-like symptoms or more severe illnesses. Moreover, alphavirus infection symptoms can be similar to those of dengue or Zika disease, leading to underreporting of cases and potential misdiagnoses. New methods that can be used to detect antibody responses to multiple alphaviruses within the same assay would greatly aid disease surveillance efforts. However, possible antibody cross-reactivity between viruses can reduce the quality of laboratory results. Our results demonstrate that antibody responses to multiple alphaviruses can be specifically quantified within the same assay by using selected recombinant protein antigens and further show that Mayaro virus infections result in unique responses to viral envelope proteins.