Metabolomic Study of a Rat Fever Model Induced with 2,4-Dinitrophenol and the Therapeutic Effects of a Crude Drug Derived from Coptis chinensis
Metabolomic Study of a Rat Fever Model Induced with 2,4-Dinitrophenol and the Therapeutic Effects of a Crude Drug Derived from Coptis chinensis
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DOI：
10.1142/s0192415x11008671
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发表时间：
2011-01-01
影响因子：
5.7
通讯作者：
Dong, Wanru
中科院分区：
文献类型：
--
作者：
Liu, Shumin;Lu, Fang;Dong, Wanru
本研究描述了发热动物模型的代谢组学及黄连提取物对发热的治疗作用。采用UPLC/ESI-Q-TOF/MS联用技术，结合主成分分析(PCA)对大鼠尿样进行分析。选择了9种离子来表征对发烧反应的相似性和差异性。墨水206.0278处的离子被明确地鉴定为黄原酸。这项研究表明，代谢组学方法可以很容易地区分发烧和健康的个体。这些数据支持这样一种观点，即代谢组学方法代表了一种很有前途的新技术，用于开发快速通过体内的发热筛查。此外，该方法还可以检测RCE的干扰效应。这项研究使作者相信代谢组学是解释中医药治疗发热的有效方法。
This study describes the metabonomics of fevers in animal models and the therapeutic effects of Rhizoma coptidis extract (RCE) on them. The rat urinary samples were analyzed by UPLC/ESI-Q-TOF/MS, combined with principal component analysis (PCA). Nine ions were chosen to characterize the similarities and differences in the responses to fever. The ion at Ink 206.0278 was unambiguously identified to be xanthurenic acid. This study demonstrated that the metabonomic approach can readily distinguish between febrile and healthy individuals. This data support the contention that the metabonomic approach represents a promising new technology for the development of rapid-throughput in vivo fever screening. Furthermore, this approach can detect the interfering effects of RCE. This investigation has led the authors to believe that metabonomics is a valid approach for explaining the therapeutic effects of traditional Chinese medicine on fevers.