Integrins are markers of human neural stem cells
Integrins are markers of human neural stem cells
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DOI：
10.1634/stemcells.2005-0595
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发表时间：
2006-09-01
期刊：
影响因子：
5.2
通讯作者：
Ffrench-Constant, Charles
中科院分区：
文献类型：
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作者：
Hall, Peter E.;Lathia, Justin D.;Ffrench-Constant, Charles
鉴定用于分离人神经干细胞（hNSCs）的标记物对于研究其生物学和治疗应用是必不可少的。本研究调查了整合素受体家族作为潜在标志物的hNSCs的表达。通过荧光激活细胞分选选择α 6（hi）β 1（hi）细胞导致人类神经前体的富集，如两种神经球形成测定和β 1（hi）群体中的神经球蛋白-1，sox 2，sox 3，巢蛋白，bmi 1和武藏1的表达增加所示。表达高水平的β 1（hi）整联蛋白的细胞也表达先前用于分离hNSC的标记物β 1（hi）蛋白-1（CD 133），并且发现使用整联蛋白β 1（hi）细胞或β 1（hi）蛋白-1（hi）细胞的选择在从神经球富集hNSC方面同样有效。因此，整合素亚基α 6和β 1由人神经前体高度表达，并代表用于其预期分离的方便标记物。
The identification of markers for the isolation of human neural stem cells (hNSCs) is essential for studies of their biology and therapeutic applications. This study investigated expression of the integrin receptor family by hNSCs as potential markers. Selection of alpha 6(hi) beta 1(hi) cells by fluorescence-activated cell sorting led to an enrichment of human neural precursors, as shown by both neurosphere forming assays and increased expression of prominin-1, sox2, sox3, nestin, bmi1 and musashi1 in the beta 1(hi) population. Cells expressing high levels of beta 1(hi) integrin also expressed prominin-1 (CD133), a marker previously used to isolate hNSCs, and selection using integrin beta 1(hi) cells or prominin-1(hi) cells was found to be equally effective at enriching for hNSCs from neurospheres. Therefore, integrin subunits alpha 6 and beta 1 are highly expressed by human neural precursors and represent convenient markers for their prospective isolation.