Bone mineral and glycosaminoglycans in newborn and mature rabbits.
Bone mineral and glycosaminoglycans in newborn and mature rabbits.
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新生和成熟兔子的骨矿物质和糖胺聚糖。
DOI：
10.1002/jbmr.5650030206
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hunter,GK
中科院分区：
文献类型：
--
作者：
Grynpas,MD;Hunter,GK
本文研究了新生兔和成熟兔骨干骨矿化与糖胺聚糖(GAG)的关系。采用密度分级技术，将骨密度从1.4克/毫升增加到2.3克/毫升，用X射线衍射仪分析每一份骨的平均晶体尺寸。采用直接萃取法测定各组分和未分离骨的GAG含量，用盐酸胍和乙二胺四乙胺顺序萃取法测定几个组分的GAG含量。GAG含量随着动物年龄的增长而降低，随着新生兔分数密度的增加而降低。在一个重叠的部分(2.0-2.1克/毫升)，新生儿的GAG含量是成熟动物的两倍。最后，从初生兔到成年兔，晶体尺寸显著增大。因此，骨骼的钙化和成熟与有机基质中蛋白多糖含量的减少有关。
The relation between bone mineralization and glycosaminoglycans (GAG) was investigated in newborn and mature rabbit diaphyseal bone. Using the density fractionation technique, the bone was separated into fractions of increasing density from 1.4 to 2.3 grams/ml. Each fraction was analyzed by X‐ray diffraction to determine the average crystal size. The GAG content of each fraction and of the unfractionated bone was determined by direct extraction and on a few fractions by sequential extraction in guanidine hydrochloride and guanidine/EDTA. There was a decrease in the GAG content with animal age and with increasing fraction density in the newborn rabbit. In one overlapping fraction (2.0–2.1 grams/ml), the GAG content was twice as high in the newborn as in the mature animal. Finally, the crystal size substantially increased from newborn to mature rabbits. Therefore, calcification and maturation of bone is associated with a decrease in the proteoglycan content of the organic matrix.