Intraoperative evaluation of sentinel lymph nodes in breast cancer: comparison of frozen sections, imprint cytology and immunocytochemistry
Intraoperative evaluation of sentinel lymph nodes in breast cancer: comparison of frozen sections, imprint cytology and immunocytochemistry
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DOI：
10.1111/j.1365-2303.2010.00818.x
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发表时间：
2011-02-01
期刊：
影响因子：
1.3
通讯作者：
Szollosi, Z.
中科院分区：
文献类型：
--
作者：
Francz, M.;Egervari, K.;Szollosi, Z.
目的：探讨印迹细胞学快速免疫细胞化学和冷冻切片分析在乳腺癌前哨淋巴结诊断中的应用价值。方法：分别计算每种方法的敏感性、特异度、阳性预测值和阴性预测值。我们将这些结果与常规石蜡切片的结果进行了比较。结果：三种方法检测转移癌的敏感性和特异性分别为：触摸印迹细胞学检测69.4%和97.8%；冷冻切片58.3%和100%；68.5%和98.9%为快速免疫细胞化学。两种方法联合使用时，触摸印迹细胞学、冷冻切片、触摸印迹细胞学加快速免疫细胞化学或触摸印迹细胞学冷冻切片分析加快速免疫细胞化学的准确性最高，灵敏度和特异性均相同，分别为72.2%和97.8%。结论：在我们的研究中，三种方法的联合准确性与触摸印迹细胞学与冷冻切片相结合或触摸印迹细胞学与快速免疫细胞化学相结合的准确性相同。快速免疫细胞化学提供了一个额外的参数和保存组织永久切片。
Objective:We analysed the utility of imprint cytology with rapid immunocytochemistry and frozen section analysis for the evaluation of sentinel lymph nodes in breast cancer patients.Methods:The sensitivity, specificity, and positive and negative predictive values have been calculated for each method individually, each pair and all three together. We compared these results with those of routinely processed paraffin sections.Results:The sensitivity and specificity of each of the three methods for detection of metastatic carcinoma were as follows: 69.4% and 97.8% for touch imprint cytology; 58.3% and 100% for frozen sections; 68.5% and 98.9% for rapid immunocytochemistry. When the methods were combined, the highest accuracy was achieved by touch imprint cytology, frozen sections, touch imprint cytology plus rapid immunocytochemistry, or touch imprint cytology frozen section analysis and rapid immunocytochemistry, each of these having identical sensitivity and specificity of 72.2% and 97.8%, respectively.Conclusions:In our study the combined accuracy of the three methods was the same as combining touch imprint cytology and frozen sections or touch imprint cytology plus rapid immunocytochemistry. Rapid immunocytochemistry provides an additional parameter and preserves tissue for permanent sections.