Determination of fluoxetine and norfluoxetine in rat plasma by HPLC with pre-column derivatization and fluorescence detection.
Determination of fluoxetine and norfluoxetine in rat plasma by HPLC with pre-column derivatization and fluorescence detection.
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采用柱前衍生和荧光检测的 HPLC 法测定大鼠血浆中的氟西汀和去甲氟西汀。
DOI：
10.1002/bmc.198
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
K. Imai
中科院分区：
文献类型：
--
作者：
Xingjie Guo;T. Fukushima;Fa;K. Imai
据报道，氟西汀（FLX）及其N-去甲基代谢产物去甲氟西汀（NFLX）均为强效的氟西汀再摄取抑制剂。一个敏感和可靠的方法，允许同时定量的血浆水平将是有价值的，并在这项工作中开发。方法：血浆中FLX和NFLX提取后，用4-（N-氯甲酰甲基-N-甲基）氨基-7-硝基-2，1，3-苯并恶二唑（NBD-COCl）进行荧光衍生化，以乙腈-水（55：45，v/v）为移动的流动相，十八烷基硅胶柱分离，荧光检测波长为537 nm，激发波长为478 nm。FLX和NFLX浓度的校准曲线在10-1000 nM范围内呈线性（r = 0.9992和r = 0.9997），100 μ L血浆中的定量限为10 nM。日内、日间精密度RSD均小于12%，日内、日间RE准确度在-6.0 ~13%之间。将所述方法应用于盐酸FLX给药大鼠血浆样品的分析和药代动力学研究。
Both fluoxetine (FLX) and its N-demethylated metabolite, norfluoxetine (NFLX), have been reported to be potent serotonin-reuptake inhibitors. A sensitive and reliable method that allows simultaneous quantification of their plasma levels would be valuable and was developed in this work. The procedure included extraction of FLX and NFLX from plasma, fluorescence derivatization with 4-(N-chloroformylmethyl-N-methyl) amino-7-nitro-2,1,3-benzoxadiazole (NBD-COCl), separation of the derivatives on an octadecylsilica column with acetonitrile-water (55:45,v/v) as mobile phase and fluorescence detection with emission at 537 nm and excitation at 478 nm. The calibration curves were linear for FLX and NFLX concentration over the range of 10-1000 nM (r = 0.9992 and r = 0.9997) and the limits of quantitation were 10 nM in 100 micro L of plasma. Precision of intra- and inter-day RSD of less than 12% and accuracy of intra- and inter-day RE within -6.0-13% were achieved. The method described was applied to analysis of the plasma samples from rats treated with FLX hydrochloride and to the pharmacokinetic study.
柱前衍生化后通过荧光检测对血浆中氟西汀和去甲氟西汀进行灵敏和选择性液相色谱分析。
影响因子：
9.3
作者：
Suckow,RF;Zhang,MF;Cooper,TB
通讯作者：
Cooper,TB