Expression of type IV collagen in different histological grades of oral squamous cell carcinoma: An immunohistochemical study
Expression of type IV collagen in different histological grades of oral squamous cell carcinoma: An immunohistochemical study
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DOI：
10.4103/0973-1482.113382
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发表时间：
2013-04-01
影响因子：
1.3
通讯作者：
Ballabh, Ram
中科院分区：
文献类型：
--
作者：
Agarwal, Pankaj;Ballabh, Ram
目的：本研究的目的是评估IV型胶原蛋白在口腔鳞状细胞癌（SCC）的三个组织学级别中的表达，并与正常口腔粘膜的表达进行比较，以确定该蛋白是否可以作为早期检测表型改变细胞生物学行为的标志物。材料与方法：由两名病理学家比较四组间IV型的染色强度、染色模式及分布模式，观察组与疾病组间差异采用卡方检验或Fisher精确检验进行统计学分析。结果：高分化(w) SCC的IV型染色强度明显高于低分化(p) SCC （p = 0.004）。与高度侵袭性SCCs相比，早期侵袭性SCCs的染色更具线性（P = 0.001）和连续性（P = 0.003）。与分化程度较低的SCC相比，其在wSCC中的分布更为连续（P = 0.003）。wSCC与pSCC的染色强度（P = 0.005）、wSCC与中分化SSC的染色强度（P = 0.003）、wSCC与pSCC的染色模式（P = 0.001）差异均有统计学意义。结论：IV型胶原的存在与SCC细胞的分化程度有直接关系，SCC细胞随着分化程度的降低而失去形成基底膜的能力。
Aim: The aim of this study was to evaluate the expression of type IV collagen in three histological grades of oral squamous cell carcinoma (SCC) in comparison with that in normal oral mucosa, to determine whether this protein can be used as a marker in early detection of the biological behavior of phenotypically altered cells.Material and Methods: The staining intensity, staining pattern, and distribution mode for type IV were compared among the four groups by two pathologists, and the differences between observers and disease groups were statistically analyzed by Chi-square test or Fisher's exact test.Results: The type IV staining intensity was more enhanced in well-differentiated (w) SCC than poorly-differentiated (p) SCC (P = 0.004). The staining was more linear (P = 0.001) and continuous (P = 0.003) in early invasive SCCs than in highly invasive SCCs. Its distribution was more continuous in wSCC than in less differentiated SCC (P = 0.003). There were statistically significant differences in the staining intensity between wSCC and pSCC (P = 0.005) or between wSCC and moderately differentiated SSC (P = 0.003) and in the staining pattern between wSCC and pSCC (P = 0.001).Conclusion: The results indicated that there was a direct relationship between the presence of type IV collagen and the differentiation degree of SCC cells and thus that SCC cells loose their capability to form the basement membrane as they become less differentiated.