Microbial cargo: do bacteria on symbiotic propagules reinforce the microbiome of lichens?
Microbial cargo: do bacteria on symbiotic propagules reinforce the microbiome of lichens?
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DOI：
10.1111/1462-2920.12658
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发表时间：
2014-12-01
影响因子：
5.1
通讯作者：
Grube, Martin
中科院分区：
文献类型：
--
作者：
Aschenbrenner, Ines Aline;Cardinale, Massimiliano;Grube, Martin
根据最近的研究，细菌作为经常性的伙伴对地衣的共生做出了贡献。然而，微生物群在物种内和地理上分散的种群之间的差异在很大程度上仍然难以捉摸。作为一种非常常见的扩散方式，地衣在特定设计的有丝分裂繁殖体中进化出真菌和藻类伙伴的垂直传播。如果细菌与这些共生繁殖菌共同传播，可能会对地衣相关微生物群的地理结构做出贡献。从奥地利东部的三个地方采集了肺地衣样本，通过条形码焦磷酸测序、网络分析和荧光原位杂交分析了它们的相关细菌群落。第一次，细菌被记录在为共生体的短距离传播而开发的地衣的共生繁殖体上。除了丝状蓝藻外，繁殖体在类群水平上与菌体具有相同的总体细菌群落结构，并以α-蛋白细菌为优势类群。所有三个采样点都共享微生物组的核心部分。同一采样点地衣菌体的细菌群落相似性高于远距离种群。这种变异以及微生物组分与宿主生物体结构的潜在共同分散为一切都是无处不在的假说提供了新的方面。
According to recent research, bacteria contribute as recurrent associates to the lichen symbiosis. Yet, the variation of the microbiomes within species and across geographically separated populations remained largely elusive. As a quite common dispersal mode, lichens evolved vertical transmission of both fungal and algal partners in specifically designed mitotic propagules. Bacteria, if co-transmitted with these symbiotic propagules, could contribute to a geographical structure of lichen-associated microbiomes. The lung lichen was sampled from three localities in eastern Austria to analyse their associated bacterial communities by bar-coded pyrosequencing, network analysis and fluorescence in situ hybridization. For the first time, bacteria were documented to colonize symbiotic propagules of lichens developed for short-distance transmission of the symbionts. The propagules share the overall bacterial community structure with the thalli at class level, except for filamentous Cyanobacteria (Nostocophycideae), and with Alphaproteobacteria as predominant group. All three sampling sites share a core fraction of the microbiome. Bacterial communities of lichen thalli from the same sampling site showed higher similarity than those of distant populations. This variation and the potential co-dispersal of a microbiome fraction with structures of the host organism contribute new aspects to the everything is everywhere' hypothesis.