Protein adsorption characterization.
Protein adsorption characterization.
复制标题
蛋白质吸附表征。
DOI：
10.1007/978-1-61779-388-2_10
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发表时间：
2012
影响因子：
--
通讯作者：
M. Barbosa
中科院分区：
文献类型：
--
作者：
M. Martins;Susana Sousa;Susana Sousa;J. Antunes;M. Barbosa
蛋白质从（水）溶液吸附到（固体）表面上是发生在生物界面处的常见过程。这种自发发生的现象改变了表面的性质，并可以诱导蛋白质的结构修饰。溶液中的蛋白质可以使用经典的生物化学方法容易地鉴定/定量。然而，吸附的蛋白质更难以评估，因为它们总是与底物结合。分析方法的选择取决于所用底物的类型、吸附蛋白质的量、溶液的类型（单一蛋白质溶液vs.复杂生物介质）和所需信息的类型（吸附蛋白质的定量、吸附动力学、构象和吸附蛋白质的方向）。到目前为止，没有一种技术能够单独表征所有的蛋白质吸附过程。因此，需要进行多技术分析。在这一章中，方法来测量人血清白蛋白聚（对苯二甲酸乙二醇酯）使用三种不同的技术，放射性标记，椭圆偏振法，石英晶体微天平与耗散- QCM-D，进行了详细描述。还介绍了与每种技术一起使用的聚合物表面的具体制备。
Protein adsorption from (aqueous) solutions onto a (solid) surface is a common process that takes place at biological interfaces. This phenomenon, that spontaneously occurs, changes the properties of the surface and can induce structural modifications on proteins. Proteins in solution can be easily identified/quantified using classical biochemical methods. However, adsorbed proteins are more difficult to assess since they are always associated with a substrate. The selection of the analytical method depends on the type of substrate used, the amount of adsorbed protein, the type of solution (single protein solution vs. complex biological media), and the type of information that is demanded (quantification of the adsorbed protein, adsorption kinetics, conformation, and orientation of the adsorbed protein). Until now, none of the techniques available are capable by its own to characterize all the protein adsorption process. Therefore, a multitechnique analysis is required. During this chapter, the methodologies to measure human serum albumin to poly(ethylene terephthalate) using the three different techniques, radiolabeling, ellipsometry, and quartz crystal microbalance with dissipation – QCM-D, are described in detail. The specific preparation of polymeric surfaces to be used with each technique is also presented.