Role of the mammalian RNA polymerase IIC-terminal domain (CTD) nonconsensus repeats in CTD stability and cell proliferation
Role of the mammalian RNA polymerase IIC-terminal domain (CTD) nonconsensus repeats in CTD stability and cell proliferation
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DOI：
10.1128/mcb.25.17.7665-7674.2005
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发表时间：
2005-09-01
影响因子：
5.3
通讯作者：
Eick, D
中科院分区：
文献类型：
--
作者：
Chapman, RD;Conrad, M;Eick, D
哺乳动物RNA聚合酶II（Pol II）的C末端结构域（CTD）由共有七肽YSPTSPS的52个重复组成，并将转录与前mRNA的加工联系起来。CTD的长度和偏离共有序列的重复序列的数量通过进化而增加，但它们的功能重要性仍然未知。在这里，我们表明，重复序列I至3或52的缺失导致CTD从Pol II在体内的裂解和降解。然而，包括这些重复序列，允许构建稳定的合成CTD。令我们惊讶的是，仅由共有重复序列组成的聚合酶可以支持细胞的正常生长和活力。我们的结论是，所有其他非共识CTD重复序列的转录和前mRNA加工的基因增殖所必需的。
The C-terminal domain (CTD) of mammalian RNA polymerase II (Pol II) consists of 52 repeats of the consensus heptapeptide YSPTSPS and links transcription to the processing of pre-mRNA. The length of the CTD and the number of repeats diverging from the consensus sequence have increased through evolution, but their functional importance remains unknown. Here, we show that the deletion of repeats I to 3 or 52 leads to cleavage and degradation of the CTD from Pol II in vivo. Including these repeats, however, allowed the construction of stable, synthetic CTDs. To our surprise, polymerases consisting of just consensus repeats could support normal growth and viability of cells. We conclude that all other nonconsensus CTD repeats are dispensable for the transcription and pre-mRNA processing of genes essential for proliferation.