Magnetic lamellar nano-hydroxyapatite as a vector for gene transfection in three-dimensional cell culture
Magnetic lamellar nano-hydroxyapatite as a vector for gene transfection in three-dimensional cell culture
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磁性层状纳米羟基磷灰石作为三维细胞培养基因转染的载体
DOI：
10.1166/jnn.2018.15365
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发表时间：
2018
影响因子：
--
通讯作者：
Wan Yizao
中科院分区：
文献类型：
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作者：
Luo Honglin;Dong Jiaojiao;Yang Zhiwei;Zuo Guifu;Li Wei;Tu Junping;Wan Yizao
与二维（2D）条件相比，在三维（3D）条件下对基因转染的研究少得多。本研究以层状纳米羟基磷灰石（L-HAp）为载体，在二维和三维细胞培养中进行了基因转染，并进行了比较。我们发现在3D条件下的转染效率比2D细胞培养高得多。此外，在2D和3D条件下，磁性增强转染效率。本研究的结果表明磁性L-HAp是一种很有前途的三维基因转染载体。
Compared to two-dimensional (2D) conditions, investigation on gene transfection in three-dimensional (3D) conditions is much less extensive. In this work, lamellar nano-hydroxyapatite (L-HAp) with and without magnetism were used as the vectors and gene transfection in 2D and 3D cell culture was carried out and compared. We found that the transfection efficiency in 3D conditions was much higher than 2D cell culture. Additionally, magnetism enhanced transfection efficiency under both 2D and 3D conditions. The findings presented in this study demonstrated that the magnetic L-HAp could be a promising vector in 3D gene transfection.