Long-lasting regeneration after ischemia in the cerebral cortex
Long-lasting regeneration after ischemia in the cerebral cortex
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DOI：
10.1161/01.str.0000252156.65953.a9
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发表时间：
2007-01-01
期刊：
影响因子：
8.3
通讯作者：
McKay, Ronald D. G.
中科院分区：
文献类型：
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作者：
Leker, Ronen R.;Soldner, Frank;McKay, Ronald D. G.
背景和目的-由于成纤维细胞生长因子2是中枢神经系统干细胞的有丝分裂原，我们探讨长期向大脑输送成纤维细胞生长因子2是否可以改善缺血后的功能结果并诱导皮质神经发生。方法-对大鼠进行永久性远端大脑中动脉闭塞，导致仅限于皮质的缺血性损伤。我们使用腺相关病毒转染系统来诱导长期成纤维细胞生长因子 2 表达并监测行为和组织学变化。结果-治疗增加了增殖细胞的数量并改善了运动行为。缺血后 90 天神经发生持续存在，并且具有神经前体和未成熟神经元特征的新生成细胞的出现在治疗后 90 天最为明显。 结论：局灶性皮质缺血引发持续的神经源性反应，成纤维细胞生长因子 2 可以增强这种反应，从而改善功能结果。
Background and Purpose-Because fibroblast growth factor 2 is a mitogen for central nervous system stern cells, we explored whether long-term fibroblast growth factor 2 delivery to the brain can improve functional outcome and induce cortical neurogenesis after ischemia.Methods-Rats underwent permanent distal middle cerebral artery occlusion resulting in an ischemic injury limited to the cortex. We used an adeno-associated virus transfection system to induce long-term fibroblast growth factor 2 expression and monitored behavioral and histological changes.Results-Treatment increased the number of proliferating cells and improved motor behavior. Neurogenesis continued throughout 90 days after the ischemia, and the occurrence of newly generated cells with characteristics of neural precursors and immature neurons was most evident 90 days after treatment.Conclusions-Focal cortical ischemia elicits an ongoing neurogenic response that can be enhanced with fibroblast growth factor 2 leading to improved functional outcome.