A new subspecies of Anoxybacillus flavithermus ssp yunnanensis ssp nov with very high ethanol tolerance
A new subspecies of Anoxybacillus flavithermus ssp yunnanensis ssp nov with very high ethanol tolerance
复制标题
具有极高乙醇耐受性的黄热厌氧芽孢杆菌云南亚种新亚种
DOI：
10.1111/j.1574-6968.2011.02294.x
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发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Peng, Hui
中科院分区：
文献类型：
--
作者：
Dai, Jun;Liu, Yang;Peng, Hui
为了寻找嗜热乙醇耐受细菌，在中国云南省的泉水，沉积物样品收集乙醇富集筛选。分离到一株新的嗜热菌E13 T。它表现出独特和显著的能力，优选在乙醇存在下生长，并且能够在60 ℃下耐受13%（v/v）乙醇。该分离物是兼性需氧、革兰氏阳性、能动的孢子形成棒，其能够利用一系列碳源，例如木糖、阿拉伯糖和纤维二糖。基于16 S rRNA基因相似性的系统发育分析表明，该菌株隶属于Anoxybacillusflavithermus（99.2%的序列相似性）。DNA-DNA杂交结果表明，E13 T与A.黄温菌DSM 2641 T.根据表型特征、系统发育数据和DNA-DNA杂交数据，认为该分离物是A. flavithermus，其名称为Anoxybacillus flavithermus ssp.云南种11月。该亚种的模式菌株为E13 T（=CCTCC AB 2010187 T =KCTC 13759 T）。
In a search for thermophilic ethanol-tolerant bacteria, water-sediment samples collected at springs in Yunnan province of China were screened by ethanol enrichment. A novel thermophilic bacterium, strain E13T, was isolated. It exhibits a unique and remarkable ability to preferably grow in the presence of ethanol and is able to tolerate 13% (v/v) ethanol at 60 degrees C. The isolate is a facultative aerobic, Gram-positive, motile, spore-forming rod that is capable of utilizing a range of carbon sources, such as xylose, arabinose and cellobiose. Phylogenetic analysis based on 16S rRNA gene similarity showed the strain to be affiliated with the species Anoxybacillus flavithermus (99.2% sequence similarity). DNA-DNA hybridization comparisons demonstrated a 64.8% DNA-DNA relatedness between strain E13T and A. flavithermus DSM 2641T. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel subspecies of A. flavithermus, for which the name Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. is proposed. The type strain of this subspecies is E13T (=CCTCC AB2010187T=KCTC 13759T).