Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE
Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE
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DOI：
10.1128/jb.180.13.3388-3392.1998
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发表时间：
1998-07-01
影响因子：
3.2
通讯作者：
Rosenbusch, JP
中科院分区：
文献类型：
--
作者：
Prilipov, A;Phale, PS;Rosenbusch, JP
已经仔细检查了大肠杆菌B-E菌株的基因组DNA，以检测迄今为止尚未鉴定的孔蛋白基因。Southern印迹分析产生了两个DNA片段，其被证明与ompC、ompF和phoE孔蛋白基因高度同源，但不同。对这两个基因进行了克隆和测序。其中之一，指定ompN，编码孔蛋白，由于低水平的表达，已经逃避了先前的鉴定。纯化至均一的OmpN孔蛋白的功能特性（单通道电导）与来自大肠杆菌的OmpC孔蛋白的功能特性（单通道电导）非常相似。coli K-12中，第二个DNA片段对应于nmpC基因，由于在其编码区插入了一个IS 1元件，该基因在大肠杆菌中不表达。从B-E开始
The genomic DNA of the B-E strain of Escherichia coli has been scrutinized to detect porin genes that have not been identified so far. Southern blot analysis yielded two DNA segments which proved highly homologous to, yet distinct fi om, the ompC, ompF, and phoE porin genes. The two genes were cloned and sequenced. One of them, designated ompN, encodes a porin which, due to low levels of expression, has eluded prior identification. The functional properties (single-channel conductance) of the OmpN porin, purified to homogeneity, closely resemble those of the OmpC porin from E. coli K-12, The second DNA fragment detected corresponds to the nmpC gene, which, due to an insertion of an IS1 element in its coding region, is not expressed in E. roll B-E.