In vitro and In Vivo evaluation of carboplatin delivery to the eye using hydrogel-iontophoresis
In vitro and In Vivo evaluation of carboplatin delivery to the eye using hydrogel-iontophoresis
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DOI：
10.1080/02713680701871140
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发表时间：
2008-03-01
影响因子：
2
通讯作者：
Pe'er, Jacob
中科院分区：
文献类型：
--
作者：
Eljarrat-Binstock, Esther;Domb, Abraham J.;Pe'er, Jacob
目的：研究体外和体内水凝胶离子电渗疗法将卡铂输送至眼睛的情况。方法：使用不同的电流强度和持续时间对类似眼睛的琼脂凝胶进行离子电渗疗法。对健康兔子进行经巩膜离子电渗疗法，施加 0、1 和 3 mA 电流 10 分钟。结果：在体外和体内研究中，无论采用何种离子电渗电流，所有实验组均获得相似的药物浓度。在琼脂模型中发现卡铂的渗透深度为 20 毫米。巩膜和视网膜中药物浓度较高，而眼液中药物浓度较低。结论：由于高扩散特性和分子电荷不足，卡铂离子电渗疗法在上述条件下的应用并不比被动渗透具有明显的优势。应进一步研究插入下侧死路的负载水凝胶中的卡铂被动扩散，作为眼内视网膜母细胞瘤的潜在临床治疗方法。
Purpose: To investigate in vitro and in vivo hydrogel-iontophoresis delivery of carboplatin to the eye. Methods: Iontophoresis was applied on agar gels resembling the eye using different current intensities and durations. Transscleral iontophoresis was performed on healthy rabbits, applying 0, 1, and 3 mA current for 10 min. Results: Similar drug concentrations were obtained in all experimental groups, in in vitro and in vivo studies, regardless of the iontophoretic current applied. A 20-mm penetration depth was found for carboplatin at the agar model. High drug levels were found at the sclera and retina, while lower levels were found at ocular fluids. Conclusion: Carboplatin-iontophoretic application at the above conditions does not have an obvious advantage over passive penetration due to high diffusion properties and insufficient molecular charge. Passive carboplatin diffusion from loaded hydrogels inserted in the lower cul-de-sac should be further investigated as a potential clinical treatment for intraocular retinoblastoma.