LATERAL INHIBITION DURING VULVAL INDUCTION IN CAENORHABDITIS-ELEGANS
LATERAL INHIBITION DURING VULVAL INDUCTION IN CAENORHABDITIS-ELEGANS
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DOI：
10.1038/335551a0
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发表时间：
1988-10-06
期刊：
影响因子：
64.8
通讯作者：
STERNBERG, PW
中科院分区：
文献类型：
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作者：
STERNBERG, PW
在秀丽隐杆线虫外阴诱导过程中，性腺的锚定细胞在一组6个多能表皮细胞（外阴前体细胞1-4）中指定了3种细胞类型的空间格局。先前的研究表明，锚定细胞产生一个渐变的感应信号，该信号可以直接刺激VPCs从基态（3型）变为1型或2型，这取决于它们与锚定细胞的距离4。在这里，我们研究了突变体lin-15中VPC之间的相互作用，其中VPC命运部分独立于感应信号5,6，并表明1型细胞积极抑制相邻细胞也成为1型细胞。因此，每个VPC的命运取决于两个细胞间信号的联合作用：来自锚定细胞的梯度感应信号和来自至少一些邻近细胞的横向抑制信号。vpcslin -15突变体的模式形成类似于昆虫早期神经发生过程中成神经细胞和皮肤细胞模式的建立7,8，这表明参与这两种模式形成的thelin-12和notchgene产物在推断分子结构上的相似性可能延伸到功能上的相似性。
DuringCaenorhabditis elegansvulval induction the anchor cell of the gonad specifies a spatial pattern of three cell types among a set of six multipotent epidermal cells, the vulval precursor cells (VPCs)1–4. Previous studies suggested that the anchor cell produces a graded inductive signal which can directly stimulate VPCs away from a ground state (type 3) to become type 1 or type 2 depending on their distance from the anchor cell4. Here, we investigate the interactions among VPCs in a mutant,lin-15, in which VPC fates are rendered partially independent of the inductive signal5,6, and show that type 1 cells actively inhibit adjacent cells from also becoming type 1 cells. The fate of each VPC therefore depends on the combined action of two intercellular signals: a graded inductive signal from the anchor cell, and a lateral inhibitory signal from at least some of its neighbours. Pattern formation among the VPCslin-15mutant is analogous to the establishment of the pattern of neuroblasts and dermatoblasts during early insect neurogenesis7,8, suggesting that the similarities in inferred molecular structure of thelin-12andNotchgene products, which are involved in these two instances of pattern formation9–12, might extend to similarities in function.