PROTECTION AGAINST INHALATION TOXICITY OF RICIN AND ABRIN BY IMMUNIZATION
PROTECTION AGAINST INHALATION TOXICITY OF RICIN AND ABRIN BY IMMUNIZATION
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DOI：
10.1177/096032719501400201
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发表时间：
1995-02-01
影响因子：
2.8
通讯作者：
UPSHALL, DG
中科院分区：
文献类型：
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作者：
GRIFFITHS, GD;LINDSAY, CD;UPSHALL, DG
1蓖麻毒素和蓖麻毒蛋白是高毒性的植物蛋白，它们在st 2中非常相似。对于相思子毒蛋白，在15只大鼠中的14只中的血清滴度在两次注射后（从实验开始6周）升高至1：12800和1：51200之间。滴度在1：256和1：1024也在用活性相思豆毒素攻击后的肺洗液中测量。3免疫血清中存在三种主要抗体类别，IgG、IgM和伊加，但在肺洗液中仅检测到IgG和伊加。肺液中伊加/IgG的比例高于血清。用相思子毒素环免疫的大鼠通过吸入可抵抗相思子毒素的5 LCt（50），但其他暴露于蓖麻毒素的大鼠则不能。4对于蓖麻毒素，两次注射后血清滴度范围为1：800至1：25600，第三次注射后滴度范围相同，但群体样品向较高滴度加权。在攻击后28天的观察期内，所有用蓖麻毒素toroid免疫的大鼠在通过吸入5LCt（50）的蓖麻毒素攻击后存活。在暴露后的不同时间取5只代表性的免疫大鼠（相思豆毒素toroid），人工杀死，并检查组织的病理变化。得出的结论是，一个明显严重的肺部病变发生在较晚的时间比非免疫，毒素攻击的大鼠。这种损伤在实验观察期内并不致命。6因此，通过皮下途径进行的免疫接种可防止吸入蓖麻毒素或相思豆毒素攻击的致死性，但不能防止显著的肺损伤。
1 Abrin and ricin are highly toxic plant proteins which are very similar in st2 Rats have been immunised against either toxin using formaldehyde-toxoids by three subcutaneous injections at intervals of 3 weeks. For abrin, serum titres in 14 out of 15 rats were raised to between 1 : 12800 and 1 : 51200 after two injections, 6 weeks from the start of the experiment. Titres of between 1 : 256 and 1 : 1024 were also measured in lung washes after challenge with active abrin toxin.3 The three major antibody classes, IgG, IgM and IgA were present in the immune sera but IgG and IgA only were detected in lung washes. The proportion of IgA to IgG was higher in the lung fluid than in sera. Rats immunised by abrin toroid were protected against 5 LCt(50)'s of abrin by inhalation but others exposed to ricin were not.4 For ricin, serum titres ranged from 1 : 800 to 1 : 25600 after two injections and after a third injection the titre range was the same but population samples were weighted towards the higher titres. All rats immunised with ricin toroid survived the challenge of 5 LCt(50)'s of ricin toxin by inhalation over the observation period of 28 days post-challenge.5 Representative immunised rats (abrin toroid) were taken at various times post-exposure, humanely killed and tissues were examined for pathological changes. It was concluded that an apparently severe lung lesion occurred at a later time than in non-immunised, toxin challenged rats. This damage was not lethal over the experimental observation periods.6 Immunisation by the sub-cutaneous route therefore protects against lethality from challenge by inhalation of ricin or abrin toxins but does not prevent significant lung damage.