Self-complementary AAVs induce more potent transgene product-specific immune responses compared to a single-stranded genome.
Self-complementary AAVs induce more potent transgene product-specific immune responses compared to a single-stranded genome.
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DOI：
10.1038/mt.2011.280
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Te-Lang Wu;Katrin Töpfer;Shih-Wen Lin;Hua Li;A. Bian;Xiangyang Zhou;K. High;H. Ertl
中科院分区：
文献类型：
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作者：
Te-Lang Wu;Katrin Töpfer;Shih-Wen Lin;Hua Li;A. Bian;Xiangyang Zhou;K. High;H. Ertl
使用小鼠模型，我们表明，与相应的单链（ss）AAV载体相比，用血清型2、7或8的衣壳假型化的自身互补（sc）腺相关病毒（AAV）载体诱导更有效的转基因产物特异性CD8+T细胞和抗体应答。这些数据表明，用scAAV载体获得的转基因产物的更高和更快速出现的量可能增加基因转移受体中的有害免疫应答。
Using a mouse model we show that self-complementary (sc) adeno-associated virus (AAV) vectors pseudotyped with capsids of serotypes 2, 7 or 8 induce more potent transgene product-specific CD8+T cell and antibody responses compared to corresponding single-stranded (ss)AAV vectors. These data suggest that the higher and more rapidly appearing amounts of transgene product achieved with scAAV vectors may increase detrimental immune responses in gene transfer recipients.