Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development
Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development
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DOI：
10.1104/pp.15.01624
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发表时间：
2016-03-01
期刊：
影响因子：
7.4
通讯作者：
Fleming, Andrew J.
中科院分区：
文献类型：
--
作者：
van Campen, Julia C.;Yaapar, Muhammad N.;Fleming, Andrew J.
叶子源自异养分生组织，在某些时候，必须通过启动光合作用过渡到自养。然而，支撑这种转换的分子和细胞过程的时间和空间协调却很少被表征。在这里，我们报告了首次建立光合能力时水稻 (Oryza sativa) 叶片发育（P3/P4 转变）特定阶段的识别。利用生理和分子相结合的方法，我们表明气孔和维管分化的元素与光合作用可测量的光吸收的开始相协调。此外，通过探索系统对环境扰动的响应，我们表明水稻叶片发育的最早阶段对于与成熟叶片光合性能相关的细胞分化元素具有显着的可塑性。最后，通过针对水稻叶片发育早期阶段进行 RNA 测序分析，我们发现了一系列基因，这些基因的表达可能支持光合作用能力的获得。我们的结果表明，当光合能力启动的几个过程协调一致时，P3/P4 转变是水稻叶片发育的一个高度动态的阶段。除了确定未来操纵水稻叶片结构/功能的基因目标外，我们的数据还强调了一个发育窗口，在此期间此类操作可能是最有效的。
Leaves are derived from heterotrophic meristem tissue that, at some point, must make the transition to autotrophy via the initiation of photosynthesis. However, the timing and spatial coordination of the molecular and cellular processes underpinning this switch are poorly characterized. Here, we report on the identification of a specific stage in rice (Oryza sativa) leaf development (P3/P4 transition) when photosynthetic competence is first established. Using a combined physiological and molecular approach, we show that elements of stomatal and vascular differentiation are coordinated with the onset of measurable light absorption for photosynthesis. Moreover, by exploring the response of the system to environmental perturbation, we show that the earliest stages of rice leaf development have significant plasticity with respect to elements of cellular differentiation of relevance for mature leaf photosynthetic performance. Finally, by performing an RNA sequencing analysis targeted at the early stages of rice leaf development, we uncover a palette of genes whose expression likely underpins the acquisition of photosynthetic capability. Our results identify the P3/P4 transition as a highly dynamic stage in rice leaf development when several processes for the initiation of photosynthetic competence are coordinated. As well as identifying gene targets for future manipulation of rice leaf structure/function, our data highlight a developmental window during which such manipulations are likely to be most effective.