Nitric oxide enhances salt tolerance in cucumber seedlings by regulating free polyamine content
Nitric oxide enhances salt tolerance in cucumber seedlings by regulating free polyamine content
复制标题
一氧化氮通过调节游离多胺含量增强黄瓜幼苗的耐盐性
DOI：
10.1016/j.envexpbot.2010.09.007
复制
发表时间：
2013-02-01
影响因子：
5.7
通讯作者：
Guo, Shi-Rong
中科院分区：
文献类型：
--
作者：
Fan, Huai-Fu;Du, Chang-Xia;Guo, Shi-Rong
一氧化氮（NO）是植物和动物体内的一种内源性信号分子，介导植物对非生物和生物胁迫的应答。以营养液为供试材料，研究了外源NO供体硝普钠（SNP）对NaCl胁迫下黄瓜生长及叶片和根系中游离多胺含量的影响。结果表明，100 μ M SNP溶液处理能显著促进NaCl胁迫下黄瓜幼苗的生长，表现为植株高度、茎粗、鲜重增加，干物质积累量增加。进一步分析表明，在NaCl胁迫下，黄瓜幼苗叶片和根系中游离多胺含量和多胺氧化酶（PAO）活性在胁迫初期显著升高，但随着胁迫时间的延长而降低。在整个处理期间，（精胺+亚精胺）/腐胺[（Spd + Spm）/Put]的值也降低了NaCl胁迫下相比，对照。而在NaCl胁迫下，施用100 μ M SNP则降低了游离Put、Spd、总游离多胺含量和PAO活性。提高了黄瓜幼苗体内Spm含量和（Spd + Spm）/Put值，调整了Put、Spd、Spm 3种游离多胺的比例。较高的（Spd + Spm）/Put值和Spm的积累有利于提高植物的耐盐性。因此，NO缓解了盐胁迫对黄瓜幼苗的伤害。NO通过调节不同类型游离多胺的含量和比例来增强黄瓜幼苗对NaCl胁迫的耐受性。(C)2010 Elsevier B. V.保留所有权利。
Nitric oxide (NO), an endogenous signaling molecule in plants and animals, mediates responses to abiotic and biotic stresses. This study was conducted in nutrient solution to investigate the effects of exogenous sodium nitroprusside (SNP), an NO donor, on plant growth and free polyamine content in cucumber leaves and roots under NaCl stress. The results showed that 100 mu M SNP in solution significantly improved the growth of cucumber seedlings under NaCl stress for 8 days, as indicated by increased, plant height, stem thickness, fresh weight and increased dry matter accumulation. Further analysis demonstrated that the content of free polyamines and the activity of polyamine oxidase (PAO) in cucumber seedling leaves and roots initially increased dramatically under NaCl stress, although they decreased over a longer period of stress. Throughout the treatment period, the value of (spermine + spermidine)/putrescine [(Spd + Spm)/Put] also decreased under NaCl stress compared to the control. In contrast, the application of 100 mu M SNP in the nutrient solution decreased the content of free Put, Spd, total free polyamines and PAO activity under NaCl stress. It also caused an increase in the content of Spm and the value of (Spd + Spm)/Put, adjusted the ratio of three kinds of free polyamines (Put, Spd, Spm) in cucumber seedlings. The high (Spd + Spm)/Put value and the accumulation of Spm were beneficial to improving the salt tolerance of plants. Therefore, NO alleviated the damage to cucumber seedlings caused by salt stress. NO enhanced the tolerance of cucumber seedlings to NaCl stress by regulating the content and proportions of the different types of free polyamines. (C) 2010 Elsevier B.V. All rights reserved.