Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells
Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells
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DOI：
10.1364/ol.35.004096
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发表时间：
2010-12-15
期刊：
影响因子：
3.6
通讯作者：
Hamaguchi, Hiro-o
中科院分区：
文献类型：
--
作者：
Okuno, Masanari;Hamaguchi, Hiro-o
我们研制了一套多焦点共焦拉曼显微光谱系统，用于活细胞的快速多模振动成像。它包括一个倒置显微镜，配备了微透镜阵列，针孔阵列，光纤束，和多通道拉曼光谱仪。采用多焦点激发和图像压缩技术，在显微镜下同时获得了48个焦点的48个拉曼光谱。多焦点共焦配置抑制了从盖玻片和细胞培养基产生的背景，使得可以在短的累积时间内获得高对比度图像。该系统使我们能够获得多模（10种不同的振动模式）的活细胞振动图像在几十秒内，只有1毫瓦的激光功率在一个焦点。这种图像采集时间比传统的单焦点拉曼显微光谱快10倍以上。(C)2010年美国光学学会
We have developed a multifocus confocal Raman microspectroscopic system for the fast multimode vibrational imaging of living cells. It consists of an inverted microscope equipped with a microlens array, a pinhole array, a fiber bundle, and a multichannel Raman spectrometer. Forty-eight Raman spectra from 48 foci under the microscope are simultaneously obtained by using multifocus excitation and image-compression techniques. The multifocus confocal configuration suppresses the background generated from the cover glass and the cell culturing medium so that high-contrast images are obtainable with a short accumulation time. The system enables us to obtain multimode (10 different vibrational modes) vibrational images of living cells in tens of seconds with only 1 mW laser power at one focal point. This image acquisition time is more than 10 times faster than that in conventional single-focus Raman microspectroscopy. (C) 2010 Optical Society of America