An amperometric hemoglobin A1c biosensor based on immobilization of fructosyl amino acid oxidase onto zinc oxide nanoparticles-polypyrrole film
An amperometric hemoglobin A1c biosensor based on immobilization of fructosyl amino acid oxidase onto zinc oxide nanoparticles-polypyrrole film
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DOI：
10.1016/j.ab.2012.08.002
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发表时间：
2012-11-15
影响因子：
2.9
通讯作者：
Pundir, Chandra Shekhar
中科院分区：
文献类型：
--
作者：
Chawla, Sheetal;Pundir, Chandra Shekhar
测量血液中的糖化血红蛋白A1c(HbA1c，糖化血红蛋白)水平可以提供糖尿病患者的长期血糖水平，而不受短期波动的影响。现有的检测HbA1c的方法，包括生物传感器，都存在灵敏度、检测限、响应时间和储存稳定性不足的问题。这些问题在目前的生物传感器中得到了解决。以纳米氧化锌/聚吡咯(ZnONPs/PPy)杂化膜为工作电极，以Ag/AgCl为参比电极，以铂(Pt)为辅助电极，将果糖基氨基酸氧化酶(FAO)固定在金(Au)电极上，构建了一种电流型HbA1c生物传感器。全血标本经溶血、酶消化后，用生物传感器检测其HbA1c水平。在+0.27V，pH 7.0，35℃的条件下，在+0.27V，pH 7.0，35℃的条件下，酶电极在2 S范围内可检测到低至50u M的果糖基缬氨酸(Fv)，线性范围为0.1~3.0 mm，灵敏度为38.42mU A mm(-1)。该电极在4℃下储存160天，初始响应仅损失30%。生物传感器测量了表面健康的人和糖尿病患者的全血中的HbA1c，发现其范围分别为4.0%至5.6%和5.7%至12.0%。(C)2012 Elsevier Inc.保留所有权利。
Measurement of hemoglobin A1c (HbA1c, glycated hemoglobin) level in blood provides the long-term glucose level in diabetic patients without the influence of short-term fluctuations. The existing methods for HbA1c determination, including biosensors, suffer from insufficient sensitivity, detection limit, response time, and storage stability. These problems were overcome in the current biosensor. A method is described for construction of an amperometric HbA1c biosensor by immobilizing a fructosyl amino acid oxidase (FAO) onto zinc oxide nanoparticles/polypyrrole (ZnONPs/PPy) hybrid film deposited onto gold (Au) electrode and using it as working electrode, Ag/AgCl as reference electrode, and platinum (Pt) as auxiliary electrode. The whole blood samples were hemolyzed and digested by protease before measuring their HbA1c level by the biosensor. The enzyme electrode detected fructosyl valine (FV) as low as 50 mu M at a signal-to-noise ratio of 3 within 2 s at +0.27 V versus Ag/AgCl, pH 7.0, and 35 degrees C with a linear working range of 0.1 to 3.0 mM for FV and sensitivity of 38.42 mu A mM(-1). The electrode showed only a 30% loss of its initial response over a period of 160 days when stored at 4 degrees C. The biosensor measured HbA1c in whole blood of apparently healthy individuals and diabetic patients and found it to be in the ranges of 4.0% to 5.6% and 5.7% to 12.0%, respectively. (C) 2012 Elsevier Inc. All rights reserved.