Secretion of YscP from Yersinia enterocolitica is essential to control the length of the injectisome needle but not to change the type III secretion substrate specificity
Secretion of YscP from Yersinia enterocolitica is essential to control the length of the injectisome needle but not to change the type III secretion substrate specificity
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DOI：
10.1111/j.1365-2958.2005.04758.x
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发表时间：
2005-09-01
影响因子：
3.6
通讯作者：
Cornelis, GR
中科院分区：
文献类型：
--
作者：
Agrain, C;Sorg, I;Cornelis, GR
耶尔森氏菌Ysc注射器的针的长度由一种称为YscP的蛋白质决定。这种蛋白质本身由注射体分泌，它既作为分子标尺又作为III型分泌的底物特异性开关。在这份报告中，我们解决了为什么YscP分泌的问题。通过系统的缺失分析和融合分子的不同部分的腺苷酸环化酶报告，我们确定了两个独立的分泌信号。其中一个包含在35个N-末端残基内，而第二个跨越残基97-137。这两种信号在功能上不同于Yop分泌信号。当两种分泌信号都被去除时，Yops仍然可以被分泌，但针长度控制丢失。只有一个信号的YscP不能很好地控制针的长度，但当更多的YscP产生和分泌时，控制得到改善。YscP剥夺了这两个信号不能控制长度，即使生产过剩。我们由此得出结论，YscP需要分泌发挥其长度控制功能，但不是其底物特异性开关功能。
The length of the needle of the Yersinia Ysc injectisome is determined by a protein called YscP. This protein, which acts both as a molecular ruler and as a substrate-specificity switch for type III secretion is itself secreted by the injectisome. In this report, we address the question why YscP is secreted. By a systematic deletion analysis and by fusing different parts of the molecule to the adenylate cyclase reporter, we identified two independent secretion signals. One of them is encompassed within the 35 N-terminal residues while the second one spans residues 97-137. These two signals are functionally different from Yop secretion signals. When both secretion signals were removed, Yops could still be secreted but the needle length control was lost. YscP possessing only one signal did not control needle length properly but the control was improved when more YscP was produced and secreted. YscP deprived of both signals could not control length, even when overproduced. We conclude from this that YscP needs to be secreted to exert its length control function but not its substrate-specificity switch function.